Complexity of Differences in Full-Length Transcriptome at Bolting Stage between Late-and Early-Bolting Chinese Cabbage Lines Revealed by Single-Molecule Nanopore DNA Sequencing Technology
To learn about the transcriptomic structure and expression pattern related to bolting in spring Chinese cabbage,the full-length transcriptomes were analyzed with samples of floral axis connected with one piece of leaf from early-bolting line He102 and late-bolting line 06-247.By aligning reads to reference genome V3.0 of Chinese cabbage,6 051 557 and 4 844 987 clean full-length mapped reads along with 37 309 and 30 415 non-redundant full-length transcripts were identified in He102 and 06-247,respectively.By integrating non-redundant full-length transcripts from the two lines,a total of 46 485 unigenes were obtained,which in-cluded 1 688 novel genes.Through alternative splicing(AS)analysis,2 421 and 993 AS events were found only occurred in He102 and 06-247 respectively,and intron retention was the most common type of the AS e-vents.He102 and 06-247 harbored 3 286 and 2 646 specific alternative polyadenylation(APA)events,re-spectively.In total,453 long non-coding RNAs(lncRNA)were identified with 81 ones only from He102 and 55 ones only from 06-247,and more than 80％of them were long intergenic non-coding RNAs.There were 5 197 differentially expressed genes were selected by comparing He 102 and 06-247,and 2 744 ones were down regulated and 2 453 ones were up regulated in He102.Combined with information from GO annotation,41 potential candidate genes related to bolting were identified.These results suggested the differences in struc-ture and expression of transcriptome between late-and early-bolting Chinese cabbage lines were very complex,and offered new information for elucidating the molecular mechanism of Chinese cabbage bolting.
The third-generation high-throughput sequencing technologySingle-molecule nanopore DNA sequencing technologyChinese cabbageBolting stageFull-length transcript
万方数据
维普
