Cloning and Expression Analysis of HD-ZIP Transcription Factor in Haidao 86
Homologous domain leucine zipper(HD-ZIP)transcription factors are unique in the plant,which are divided into four different subfamilies(HD-ZIP Ⅰ to Ⅳ).HD-ZIP Ⅰ play key roles in regulation of plant development processes,signal networks and response to environmental stresses.Haidao 86 is a specific rice germplasm with high saline-alkali tolerance.In order to analyze the function of HD-Zip Ⅰ in response to salt stress,an HD-ZIP Ⅰ gene named OsHDZ1 was cloned by RT-PCR technology using Haidao 86 as materi-al,and its bioinformatics analysis,subcellular localization and expression analysis under salt stress were con-ducted.The results showed that the CDS sequence of OsHDZ1 gene was 831 bp in length,encoding 276 amino acids,with the molecular weight of 30.65 kDa and the theoretical isoelectric point of 5.19,which was a hydro-philic unstable protein.OsHDZ1 protein contained no transmembrane structure,contained 25 phosphorylation sites,and the secondary structure mainly consisted of α-helix and random coil.Homology analysis showed that its protein sequence was highly related to Eragrostis curvula and Triticum aestivum.The prediction of the pro-moter element of OsHDZ1 showed that it had hormone(such as abscisic acid and jasmonic acid)response ele-ments,as well as response elements to environmental stresses.Subcellular localization analysis showed that the protein was localized in the nucleus.According to the results of fluorescence quantitative PCR,the expression of OsHDZ1 was significantly up-regulated and gradually increased in the former 60 hours of salt stress,and de-creased at 72 hours,but the overall trend was still on the rise,indicating that this gene might have the regula-tory function in salt tolerance of Haidao 86.The results of this study laied the foundation for subsequent in-depth analysis of the function of OsHDZ1 gene in regulating salt tolerance of Haidao 86,and could provide gene resources for breeding salt-tolerant crops.
万方数据
维普
