Effects of Adding Different Organic Nitrogen Sources into PDA Medium on Rejuvenation of Auricularia auriculata Degraded Strain
In order to solve the problem of stain degradation in cultivation and production process of black fungus(Auricularia auriculata),it is necessary to develop an effective method of fungus rejuvenation to enhance the viability of mycelia.In this experiment,the degraded black fungus strain was used as research ob-ject;the effects of adding six organic nitrogen substances,including corn flour,peptone,spirulina powder,malt extract,beef extract and yeast extract,on the mycelial growth rate,biomass,contents of nutrients and active oxygen,antioxidant enzymes activities of degraded black fungus mycelia in PDA medium were studied.The results showed that compared with PDA medium(CK),adding spirulina powder and malt extract made the mycelia grow faster,the biomass be higher,and the contents of protein and polysaccharide in mycelia in-crease significantly,but reduced the contents of 02·and H2O2 in mycelia significantly except the H202 content in mycelia with the addition of malt extract.The activities of peroxidase(POD),superoxide dismutase(SOD)and catalase(CAT)in mycelia were significantly increased when adding spirulina powder compared with CK.To sum up,the optimal culture medium for rejuvenating the degraded strains of black fungus was PDA culture medium with spirulina powder added.This study could provide technical support and theoretical references for rejuvenating the aging and degraded black fungus strains.
万方数据
维普
