Physiological Metabolism and Transcriptomic Analysis of Cyperus esculentus L.Seedlings under Alkali Stress
Cyperus esculentus L.is both an herb oil crop and an applied cash crop for grain,oil,feed and fertilizer with high comprehensive utilization value,furthermore,it has the characteristics of drought re-sistance and salt and alkali tolerance.Identifying the molecular regulation mechanism of alkali resistance of C.esculentus could lay a foundation for its molecular breeding.In this study,the seedlings of C.esculentus were subjected to 100 mmol/L mixed alkali(NaHCO3 and Na2CO3 at 2:1 of mole ratio)stress treatment,and were sampled at 0,3,6,9 and 12 days after treatment.Physiological indicators such as contents of malondialde-hyde(MDA),soluble protein(SP)and proline(Pro),and activities of superoxide dismutase(SOD),per-oxidase(POD)and catalase(CAT)were determined,and transcriptome analysis was performed.The results showed that under alkali stress,SOD and CAT activities increased rapidly in the earlier 6 days and then de-creased gently;POD activity increased rapidly first and then decreased rapidly after 6 days of stress;MDA content increased gradually first and increased rapidly after 6 days;both Pro and SP contents showed gradual upward trends,and the former increased rapidly,while the latter increased relatively slowly.Transcriptome a-nalysis showed that 5 842 differentially expressed genes(DEGs)were identified at 6 days of alkali stress,a-mong which,3 136 genes up-regulated and 2 706 genes down-regulated.GO and KEGG enrichment analysis showed that the DEGs were significantly enriched at the processes of plant hormone signal transduction,starch and sucrose metabolism and so on.In addition,the results of real-time fluorescence quantitative PCR(qRT-PCR)analysis were roughly the same as those of transcriptome analysis,which confirmed the validity of the RNA-seq data.The above results provided important information for further understanding the response mecha-nism of C.esculentus seedlings to alkali stress.
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