球毛壳菌ND35对盐胁迫下辣椒幼苗生长和生理特性的影响
Effects of Chaetomium globosum ND35 on Growth and Physiological Characteristics of Pepper Seedlings under Salt Stress
孟庆果 1李玲 2高广增 3孙帅斌 4闫学成 4高克祥4
作者信息
- 1. 沂水县公共资源交易中心,山东 沂水 276400;山东农业大学植物保护学院/山东省蔬菜病虫生物学重点实验室,山东 泰安 271018
- 2. 山东农业大学植物保护学院/山东省蔬菜病虫生物学重点实验室,山东 泰安 271018;肥城市农业农村局,山东 肥城 271600
- 3. 山东农业大学植物保护学院/山东省蔬菜病虫生物学重点实验室,山东 泰安 271018;青岛林业植物医院工程有限公司,山东 青岛 266100
- 4. 山东农业大学植物保护学院/山东省蔬菜病虫生物学重点实验室,山东 泰安 271018
- 折叠
摘要
为给球毛壳菌(Chaetomium globosum)ND35 在盐碱地蔬菜生产中的应用提供技术依据,本试验以辣椒幼苗材料,采用浇灌不同浓度盐溶液(0、50、100、150 mmol·L-1 NaCl)方法模拟盐胁迫,研究接种内生真菌球毛壳菌ND35 对盐胁迫下辣椒幼苗生长和生理特性的影响.结果表明:盐胁迫下,与不接种球毛壳菌ND35 的处理(CK)相比,接种球毛壳菌ND35 能提高辣椒幼苗地上部、地下部鲜重及根冠比;0、50、100、150 mmol·L-1 NaCl胁迫下接种球毛壳菌ND35 处理辣椒叶片的可溶性蛋白含量较CK分别提高 16.05%、9.11%、17.51%和6.38%,丙二醛含量分别降低 15.59%、6.68%、13.68%和 18.02%,脯氨酸含量分别降低 26.93%、36.48%、33.28%和 50.80%,抗氧化酶(SOD、POD、CAT)活性都高于CK,且NaCl浓度为 150 mmol·L-1 时辣椒叶片的SOD活性比CK提高 126.86%.综之,接种内生真菌球毛壳菌ND35 能够提高盐胁迫下辣椒幼苗的耐受性,保护其健康生长.
Abstract
In order to provide technical basis for the application of Chaetomium globosum ND35 in pro-duction of vegetables in saline-alkali soil,pepper seedlings were used as materials in the test,and the effects of inoculation with endophytic fungus C.globosum ND35 on growth and physiological characteristics of pepper seedlings under salt stress were studied by irrigating different salt solution(0,50,100 and 150 mmol·L-1 NaCl)to simulate salt stress.The results showed that under salt stress,inoculation with C.globosum ND35 could improve the fresh weight above and under ground as well root-shoot ratio of pepper seedlings compared with the control(CK)without inoculation.Under stress of 0,50,100 and 150 mmol·L-1 NaCl,the soluble protein content in leaves inoculated with C.globosum ND35 increased by 16.05%,9.11%,17.51%and 6.38%,respectively compared with CK;the malondialdehyde(MDA)and proline(Pro)contents decreased by 15.59%,6.68%,13.68%and 18.02%,and 26.93%,36.48%,33.28%and 50.80%,respectively;the activities of antioxidant enzymes(CAT,POD,SOD)were higher than that of CK,and the SOD activity in-creased by 126.86%when the NaCl concentration was 150 mmol·L-1.In conclusion,inoculation with the en-dophytic fungus C.globosum ND35 could enhance tolerance of pepper seedlings under salt stress and protect their healthy growth.
关键词
内生真菌/球毛壳菌ND35/辣椒/盐胁迫/生理特性Key words
Endophytic fungus/Chaetomium globosum ND35/Pepper/Salt stress/Physiological char-acteristics引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据