Pathogen Identification for Maize Seedling Blight and Their Sensitivity to Tebuconazole
To clarify the types,pathogenicity and sensitivity to tebuconazole of pathogens causing maize seedling blight,this study focused on the maize plants infected by maize seedling blight and collected from Zi-bo City,Shandong Province in 2021.The pathogens were isolated,identified and tested for pathogenicity and sensitivity to tebuconazole.The results showed that 16 strains were isolated from the roots of maize seedlings with blight.Through morphological observation,sequence alignment and phylogenetic analysis,these strains were identified including 7 strains of Fusarium oxysporum,4 strains of F.incarnatum-equiseti species complex,2 strains of F.pseudograminearum,2 strains of F.solani,and 1 strain of F.tricinctum.Pathogenicity tests re-vealed that F.oxysporum,F.incarnatum-equiseti species complex and F.pseudograminearum could cause root disease in maize seedlings.Sensitivity test to tebuconazole showed that the EC50 value of the 16 strains ranged from 0.03 μg/mL to 3.18 μg/mL,and two strains had the EC50 value higher than 0.30 μg/mL.In conclusion,the dominant pathogens of maize seedling blight were F.oxysporum and F.incarnatum-equiseti species complex in Zibo City,Shandong Province.Additionally,the strains with reduced susceptibility to tebuconazole were found in this study,suggesting the use of tebuconazole in complex with other fungicides to control maize seed-ling blight so as to extend the effective lifespan of tebuconazole.
国家科技期刊平台
NSTL
万方数据
