Molecular Identification and Comparison on Biological Characteristics of Six Walnut Canker Pathogens
In order to investigate the specific types and biological characteristics of walnut canker patho-gens,six isolates of walnut canker pathogen with discrepant colony morphologies were jointly identified with multiple genes based on molecular biological methods,and the variations in their pathogenicity and biological characteristics were further explored.The results indicated that based on the phylogenetic tree,the strain 44-9-2B was clustered with Cytospora melnikii with the bootstrap support rate of 100%;the strains 44-1-2C and 51-1-3C were clustered with C.chrsosperma;the strains 49-2-1A,49-9-4D and 53-1-3F were clustered with Valsa sordida.The strain 44-9-2B had the strongest pathogenicity and produced conidia first,followed by strains 44-1-2C and 51-1-3C,while strains 49-2-1A,49-9-4D and 53-1-3F were the last.The results of biological char-acteristics showed that the optimal growth temperature for the six pathogenic strains was 28℃,and the optimal pH value was 6;light had promoting effects on the growth of the six pathogenic strains;but the optimal nitrogen and carbon sources were different for different pathogenic strains.The findings of this study could provide a theoretical basis for prevention and control of the walnut canker pathogens.
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