Isolation and Identification of Bacillus Degrading Aflatoxin B1 and Its Characterization
The aim was to isolate Bacillus sp.with degrading effect on aflatoxin B1(AFB1)from samples of different sources,so as to provide a theoretical basis for the exploitation and utilization of Bacillus.strains degrading AFB1.The intestinal contents and anal swabs of chickens and soil samples were collected.After en-richment,the suspected Bacillus colonies were selected through culture using MYP and primary screening me-dium,and then the strains with degrading effect on AFB1 were selected by analyzing the degradation rate of AFB1 and identified the bacterial genus and species by 16S rRNA sequencing.The selected Bacillus strains were tested for hemolysis,antibiotic sensitivity,growth curve,acid and bile salt resistance.The results showed that a total of 20 Bacillus strains degrading AFB1 were selected.Combined with the results of bacterial sequen-cing and hemolysis test,three B.licheniformis strains(S51,S48,S8-2)with high degradation rate were se-lected for further tests.The results showed that the three B.licheniformis strains were sensitive to multiple anti-biotics;among which,S51 had the highest growth rate,the largest concentration of bacterial fluid,and was more tolerant to both acid and bile salt than the other two strains.Overall,one B.licheniformis strain with bet-ter degrading effect on AFB1 was selected for its good safety and tolerance,which was expected to be applied as a microecological agent in the degradation process of AFB1.
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