Effects of Exogenous Nitric Oxide on Seed Germination and Seedling Physiological Characteristics of Maize under NaCl Stress
To explore the mitigation effect of exogenous nitric oxide(NO)on inhibition of salt stress to maize seed germination and seedling growth,using the variety Longdan 339 seeds as test material,the experi-ment was conducted to study the effects of different concentrations(0.1,0.2,0.5,1.0,2.0 mmol/L)of sodi-um nitroprusside(SNP,NO donor)on seed germination,seedling growth and physiological properties of maize under 100 mmol/L NaCl stress.The results showed that the germination rate,germination potential,vig-or index and germination index of seeds,and root length,bud length,root fresh weight and bud fresh weight of seedlings under NaCl stress treatment(condition control)were significantly reduced compared with the blank control(CK);the superoxide dismutase(SOD)and peroxidase(POD)activities in roots and shoots increased significantly,and the catalase(CAT)activity decreased significantly;the contents of soluble sug-ars,proline and malondialdehyde(MDA)increased significantly.After soaking seeds with suitable concentra-tions of SNP,the seed germination characteristics and seedling growth related indicators were significantly im-proved compared with NaCl stress treatment,the activities of CAT,SOD and POD in roots and buds were sig-nificantly improved,the accumulation of proline and soluble sugars were further significantly promoted,and the MDA content was significantly reduced.Among which,soaking seeds with 0.2 mmol/L SNP had the best effect,which significantly enhanced the resistance of maize seeds and seedlings to salt stress.These results could provide the theoretical basis and data support for the improvement of salt resistance at maize germination stage.
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