Comparison of breast cancer bone metastasis models constructed by three injection methods of breast cancer cells
Objective To compare the success rate and pathological status of breast cancer bone metastasis models constructed by injections of breast cancer cells through left ventricle,tibial bone marrow cavity and tail artery.Methods Twenty-four BALC/c nude mice were randomly divided into the left ventricular injection group,tibia injection group,and tail artery injection group,with 8 in each group.Human breast cancer cells MDA-MB-231-luc were injected into the left ventricle,tibia,and tail artery,respectively,to build bone metastasis models of breast cancer.Sixteen BALC/c mice were randomly divided into the left tibia injection group and tail artery injection group,with 8 mice in each group.The mouse-derived breast cancer cells 4T1-luc were injected into tibia bone marrow cavity and tail artery,respectively,to con-struct bone metastasis models of breast cancer.Small Animal In Vivo Imaging was used to observe the model establishment and the occurrence of bone metastasis.The pathological changes of tumor tissues were observed by HE staining.Results① One mouse died in the left ventricular injection group of MDA-MB-231-luc cells,while all mice from other modeling methods survived.The results of Small Animal In Vivo Imaging and HE staining showed that the success rate of modeling in the left ventricular injection group was around 60%.On the 20th day after modeling in nude mice,fluorescence signals were scattered throughout the entire body,with a small number of cancer cells infiltrating bone tissue and minimal bone de-struction.In the tibial injection group,fluorescence signals of tumor cells in both tibia were observed,with significant bone destruction and clustered growth of tumor cells.The tail artery injection group showed fluorescence signals of tumor cells in both lungs,and no obvious bone metastases were observed.② After injection of 4T1-luc cells,all mice survived.On the 10th day after modeling,Small Animal In Vivo Imaging was used to display strong fluorescence signals of tumor cells.The cancer cells in the tail artery injection group were implanted in both femurs and the back of the tail,and the can-cer cells grew around the bone tissue,continuously eroding the bone.The fluorescence signal imaging of tumor cells in the tibia injection group was on both sides of the tibia,with a large number of breast cancer cell infiltration which showed out-ward growth;the tumor cells had abnormal morphology and were tightly arranged,with a small amount of patchy bone tis-sue visible in the tumor lesion.Conclusions The use of breast cancer cells MDA-MB-231-luc and 4T1-luc for left ven-tricular injection,tibial injection,and tail artery injection can all induce significant bone metastasis,but there are certain differences in the success rates and pathological status of modeling.The success rate of tibial injection is the highest,while left ventricular injection and tail artery injection can simulate the process of bone metastasis in the early stage.
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