Notch4 mRNA expression in oropharyngeal squamous carcinoma tissues and its effects on cell proliferation,migration,and invasion
Objective To observe the expression changes of Notch4 mRNA in tissues and cells of oropharyngeal squamous cell carcinoma,and to explore the effect of knocking down Notch4 mRNA expression on the proliferation,migra-tion,and invasion abilities of oropharyngeal squamous cell carcinoma cells.Methods We collected 12 pairs of oropha-ryngeal squamous cell carcinoma tissues and adjacent tissues,and detected Notch4 mRNA expression levels in the cancer tissues and adjacent tissues by RT-qPCR.We cultivated oropharyngeal squamous cell carcinoma cell lines Detroit 562 and WSU-HN-30,and control cells HaCaT,2BS to detect Notch4 mRNA.The cell lines Detroit 562 and WSU-HN-30 were randomly divided into the si-NC group(transfected with si-NC double stranded nucleotide),si-Notch4-1 group(transfect-ed with si-Notch4-1 double stranded nucleotide),and si-Notch4-2 group(transfected with si-Notch4-2 double stranded nu-cleotide).Notch4 mRNA was detected in cells of each group,and the proliferation ability(CI value)of cells in each group was observed using RTCA method.The invasion and migration abilities of cells in each group were observed by Transwell experiment.Results Compared with the adjacent tissues,the relative expression level of Notch4 mRNA in oropharyn-geal squamous cell carcinoma tissues was higher(P<0.01).There was no difference in the relative expression level of Notch4 mRNA between HaCaT and 2BS cells in the control group.Compared with HaCaT cells in the control group,the relative expression levels of Notch4 mRNA were higher in the Detroit 562 and WSU-HN-30 cells(both P<0.01).In the Detroit 562 and WSU-HN-30 cells,compared with the si-NC group,the cell proliferation,migration,and invasion abili-ties of the si-Notch4-1 and si-Notch4-2 groups decreased(all P<0.001).Conclusion Notch4 was highly expressed in oropharyngeal squamous carcinoma tissues and cells,and knockdown of Notch4 expression significantly inhibited the pro-liferation,migration and invasion of oropharyngeal squamous carcinoma cells.
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