Immune cell distribution in rectal cancer tissues based on the data of gene detection in GEO database
Objective Based on the expression profile sequencing data of rectal cancer tissues from the GEO data-base,the study investigated the proportion of immune cells in rectal cancer tissues and analyzed the relationships between different immune cells and clinicopathological features and prognosis,so as to investigate the distribution of immune cells in rectal cancer and its clinical significance.Methods The GSE87211 dataset was downloaded from the GEO database to extract gene detection data of rectal cancer tissues and normal tissues and the clinical data of rectal cancer patients.The proportion of 22 kinds of immune cells in rectal cancer and normal tissues was calculated by CIBERSORT deconvolution method(including Plasma cells,naïve B cells,memory B cells,follicular helper T cells,regulatory T cells,γ δ T cells,CD8+ T cells,naïve CD4+ T cells,resting CD4 memory T cells,activated CD4 memory T cells,resting NK cells,resting mast cells,activated mast cells,eosinophils,neutrophils,activated NK cells,monocytes,M0 macrophages,M1 macro-phages,M2 macrophages,resting dendritic cells and activated dendritic cells).Pearson correlation analysis was used to analyze the correlation between different immune cells;Wilcox test or Kruskal-Wallis test were used to compare the propor-tions of immune cells in cancer tissues of colorectal cancer patients with different clinicopathologic characteristics.The pa-tients with rectal cancer were divided into the high proportion group and low proportion group according to the median pro-portion of different immune cells.Kaplan-Meier curve was used to compare the overall survival differences between high and low proportion groups of different immune cells,and the survival rates of the two groups were compared.Results Compared with the normal tissues,the proportions of naïve CD4+ T cells,activated CD4 memory T cells,regulatory T cells,resting NK cells,monocytes,M0 macrophages,resting dendritic cells,activated mast cells and neutrophils in rec-tal cancer tissues increased,and the proportions of memory B cells,CD8+ T cells,plasma cells,resting CD4 memory T cells,follicular helper T cells,M2 macrophages and resting mast cells decreased in the rectal cancer patients(all P<0.05).The proportions of activated mast cells and neutrophils had the strongest positive correlations(r= 0.71),the pro-portions of M2 macrophages and plasma cells were positively correlated(r=0.65,P<0.05),and the proportion of CD8+ T cells was also positively correlated with the proportion of plasma cells(r=0.62).The the proportion of neutrophils was neg-atively correlated with the proportion of M2 macrophages(r=-0.62).Compared with patients with KRAS wild-type rectal cancer,the proportions of activated CD4 memory T cells and neutrophils increased,and the proportion of regulatory T cells increased in patients with KRAS mutant rectal cancer(all P<0.05).The proportions of plasma cells,γ δ T cells and regu-latory T cells in the rectal cancer patients with lymph node metastasis were lower than those in patients without lymph node metastasis;the proportion of resting NK cells in patients with distant metastasis was higher than that in patients without me-tastasis(all P<0.05).Patients with high proportion of plasma cells had better survival prognosis;the survival of patients with high proportion of neutrophils was significantly shorter than that of patients with low proportion(all P<0.05).Con-clusions The distribution of immune cell in rectal cancer tissues is different from that in the normal tissues,and the pro-portion of immune cell subsets is different in patients with different clinicopathological features of rectal cancer.Patients with high expression of plasma cells indicate a better survival outcome,and high expression of neutrophils is associated with poor clinical prognosis.
immune cellstissue immune cellstissue infiltration of immune cellstumor microenvironmentrec-tal cancerprognosis of tumor
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