Application value of infrared thermal imaging in diagnosis of venous thromboembolism in rabbits
Objective To investigate the application value of infrared thermal imaging in venous thromboembolism in rabbits.Methods Sixty male New Zealand white rabbits were selected and fed adaptively for 1 week.They were random-ly divided into the T0 group,T1 group,T2 group and T3 group,with 15 rabbits in group.The animal models of blood stasis syndrome were prepared in the T1 group,T2 group and T3 group,in which T1 group was made once on the first day of the experiment,T2 group was made once each on the first and second days of the experiment,and T3 group was made once each on the first,second and third days of the experiment.The animal model of blood stasis syndrome was not prepared in the T0 group,only two hind limbs were covered.Infrared thermography was used to detect the temperature difference between the two hind limbs at 6 h after skin preparation in the T0 group and 6 h after the last molding in the T1,T2 and T3 groups.The internal diameter and blood flow of the femoral vein of the right hind limb were measured by color Doppler ultrasonography.Blood was collected from abdominal aorta,serum was collected by centrifugation,and coagulation function[fibrinogen(FIB),antithrombin Ⅲ(AT-Ⅲ),D-dimer(D-dimer)]and inflammatory factors[interleukin(IL)-1β,IL-6,superoxide dismutase(SOD),and tumor necrosis factor-α(TNF)]were detected by ELISA.NO test box(one-step method)was used to detect serum NO.The deep vein and pulmonary artery of the right hind limb were dissected.The thrombosis of the deep vein and pulmonary artery of the right hind limb was observed and their length was measured.Results The temperature difference distribution of the two hind limbs was as follows:T0 group<T1 group<T2 group and T3 group(all P<0.05),but there was no statistical difference between T2 group and T3 group(P>0.05).The internal diameter and blood flow of femoral vein in the right hind limb were as follows:T0 group>T1 group>T2 group>T3 group(all P<0.05).FIB was as follows:T0 group<T1 group<T2 group<T3 group(all P<0.05);AT-Ⅲ was as follows:T0 group,T1 group>T3 group,T1 group>T2 group(all P<0.05);D-Dimer was as follows:T0 group,T1 group<T2 group<T3 group(all P<0.05).There were no significant differences in serum levels of IL-1β,IL-6,SOD or TNF-α among all groups(all P>0.05).NO was as follows:T0 group,T1 group<T2 group,T3 group(P<0.05).The length of deep vein thrombosis in the right hind limb was as follows:T0 group<T1 group<T2 group and T3 group(all P<0.05),but there was no statistically significant difference between T2 group and T3 group(P>0.05).The length of pulmonary artery thrombosis was as follows:T0 group,T1 group<T2 group and T3 group(P<0.05),but there was no statistically significant difference between T0 group and T1 group,T2 group and T3 group(all P>0.05).Conclusion Infrared thermal imaging is valuable in the diagnosis of venous thromboembolism in rabbits,and can be used as an auxiliary diagnostic means.
venous thromboembolisminfrared thermal imaging technologycoagulation functioninflammatory factorbig white rabbit
万方数据
维普
