Intervention effect of Drp1 inhibitor on ischemia-reperfusion injury in intestinal mucosal epithelial cells
Objective To observe the intervention effect of dynamin-related protein 1(Drp1)inhibitor on ischemia-reperfusion injury of intestinal mucosal epithelial cells and to analyze its mechanism.Methods Human colorectal muco-sal epithelial cells Caco-2 were divided into the control group,model group,and Drp1 inhibitor group,respectively.The cells in the control group were cultured normally.In the model group and Drp1 inhibitor group,hypoxia-reperfusion mod-els were constructed by using the method of hypoxia for 12 h followed by reoxygenation for 2 h;cells in the Drp1 inhibitor group were given the intervention of the Drp1 inhibitor-Mdivi-1 before the H/R treatment.Cell viability was detected by CCK-8 assay,mitochondrial reactive oxygen species(ROS)content was detected by mitochondrial superoxide indicator,mitochondrial membrane potential level was detected by JC-1 assay,apoptosis rate was detected by flow cytometry,and the expression levels of Drp1,and mitochondrial fusion protein 2(mitofusin2,Mfn2)were detected by Western blotting.Results Cell viability was as follows:control group>Drp1 inhibitor group>model group(all P<0.05),intracellular mitochondrial ROS content was as follows:model group>Drp1 inhibitor group>control group,mitochondrial membrane potential was as follows:control group>Drp1 inhibitor group>model group(all P<0.05),apoptosis rate was as follows:model group>Drp1 inhibitor group>control group(all P<0.05),cellular Drp1 protein expression was as follows:model group>Drp1 inhibitor group>control group,and Mfn2 protein expression was as follows:control group>Drp1 inhibitor group>model group(all P<0.05).Conclusion Drp1 inhibitor could reduce ischemia-reperfusion injury in intestinal mucosal epithelial cells,and its mechanism might be related to improving mitochondrial dysfunction and reducing apopto-sis.
dynamin-related protein 1intestinal ischemia-reperfusion injurymitofusion 2mitochondrial func-tionmitochondrial dynamics
万方数据
维普
