Therapeutic effect of intragastric administration of Buqi Tongqiao formula on allergic rhinitis in rats and its mechanism
Objective By establishing rat models of allergic rhinitis(AR),the therapeutic effect of Buqi Tongqiao formula(BQTQF)on AR rats was observed and its mechanism was analyzed.Methods Wistar rats were randomly divid-ed into four groups:control group,model group,BQTQF group,and Biyankang group,with 10 rats in each group.Except the control group,AR models were established with the suspension of ovalbumin antigen adjuvant in the other groups.Af-ter modeling,rats in the the model group were administered intragastrically 4 mL/d pure water,rats in the BQTQF group were administered 57.55 g/(kg·d)of BQTQF intragastrically,and rats in the Biyankang group were given 462.5 mg/(kg·d)of Biyankang intragastrically,while rats in the control group were fed routinely without gavage.The symptoms of nasal scratching,sneezing and runny nose were observed within 30 min after the end of gavage and the ethological scores were calculated;the pathological changes of nasal mucosa in rats with AR were observed by HE staining;Western blotting was used to detect NF-κB p65 protein in spleen;16S rRNA gene sequencing was used to detect the diversity and abundance of intestinal flora.Results The scores of animal behavior in the model group,BQTQF group and Biyankang group were higher than those in the control group.After 2 days of intragastric administration,the scores of BQTQF group were lower than those of the model group.After 3 days of intragastric administration,the scores of Biyankang group were lower than those of the model group(all P<0.05).In the control group,there were clear nasal mucosa pseudostratified ciliated colum-nar epithelium,but there were no pathological changes such as edema and congestion in the lamina propria.In the model group,the nasal mucocilia were disordered,and more inflammatory cells such as eosinophils were infiltrated and the num-ber of glands increased.The lamina propria mucosae of BQTQF group and Biyankang group were relatively intact,the de-gree of inflammatory cell infiltration was reduced in comparison with that of the model group;there was no obvious glandu-lar hyperplasia,and the pathological condition of nasal mucosa was improved.Spleen NF-κB p65 protein expression was as follows:model group>BQTQF group,Biyankang group>control group(all P<0.05).The control group,model group,BQTQF group and Biyankang group contained 3 687,1 641,1 222 and 891 species of microflora,and there were differences in microflora structure between groups.At the level of bacterial genus,there were significant differences in the specific bacterial genera of intestinal flora among all groups.At the family level,relative abundance of Lactobacillaceae was in the following order:control group>BQTQF group,Biyankang group>model group(P<0.05).Conclusion BQTQF can improve the symptoms of rhinitis and repair the pathological injury of nasal mucosa in AR rats,and its mecha-nism may be related to increasing the relative abundance of Lactobacillaceae,regulating intestinal microecology,and down-regulating the expression of NF-κB P65 protein in spleen.
万方数据
维普
