Sensitization effect and mechanism of Brucea Javanica oil emulsion on cisplatin chemotherapy in lung cancer A549 cells
Objective To observe the sensitization effect of Brucea Javanica oil emulsion on cisplatin chemotherapy in lung cancer A549 cells and to analyse its mechanism.Methods Human lung cancer cells A549 were cultured in vi-tro.MTT method was used to detect the inhibitory rates of Brucea Javanica oil emulsion,cisplatin,Brucea Javanica oil emulsion combined with cisplatin on cell growth.The half inhibitory concentration(IC50)of Brucea Javanica oil emulsion and cisplatin on cells was calculated,and the effects of different concentrations of Brucea Javanica oil emulsion(2.44,9.77,78.12 μg/mL)on cisplatin IC50 were observed.A549 cells were divided into four groups:the control group,Brucea Javanica oil emulsion group,cisplatin group,and Brucea Javanica oil emulsion + cisplatin group.Cells in the control group were routinely cultured,while cells in the Brucea Javanica oil emulsion group,cisplatin group,and Brucea Javanica oil emulsion+cisplatin group were treated with Javanica oil emulsion,cisplatin,Brucea Javanica oil emulsion and cisplat-in,respectively.Fluorescence microscopy was used to observe cell morphology and growth,flow cytometry was used to de-tect cell cycle and apoptosis rate,and dichlorofluorescein diacetate fluorescent probe staining was used to detect intracellu-lar reactive oxygen species(ROS)levels.Results The IC50 of cisplatin decreased with the increase of the concentrations of Brucea Javanica oil emulsion(all P<0.05).In the control group,cells grew well,there were a larger number of cells,with a normal growth state in shape.The number of cells in the cisplatin group and the Brucea Javanica oil emulsion group decreased in comparison with that of the control group,with a small number of debris visible.In the Brucea Javanica Ja-vanica oil emulsion and cisplatin group,the cell growth was significantly inhibited,the number of cells was the smallest,the shape of cells was irregular and the cell debris increased.The proportion of cells in the G0/G1 phase was as follows:Brucea Javanica oil emulsion + cisplatin group>cisplatin group,Brucea Javanica oil emulsion group>control group,the apoptosis rate was as follows:Brucea Javanica oil emulsion + cisplatin group>Brucea Javanica oil emulsion group,cisplat-in group>control group,and the intracellular ROS levels were as follows:Brucea Javanica oil emulsion + cisplatin group>Brucea Javanica oil emulsion group,cisplatin group>control group(all P<0.05).Conclusion Brucea Javani-ca oil emulsion can increase the sensitivity of lung cancer A549 cells to cisplatin chemotherapy,and its mechanism may be related to blocking tumor cells in G0/G1 phase,increasing the production of ROS,and promoting the apoptosis.
Brucea Javanica oil emulsionlung carcinomadrug resistance in tumorchemosensitivitycisplatin
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