Regulation of tumor cell exosomes with high and low expression of miR-296-5p on migration and invasion of ESCC cells
Objective To explore the regulatory effect and mechanism of tumor cell exosomes with high and low ex-pression or miR-296-5p on the metastasis of esophageal squamous cell carcinoma(ESCC)cells.Methods The exo-somes of ESCC cells transfected with miR-296-5p overexpression,miR-296-5p interference and its negative control(NC)were extracted,and identified by transmission electron microscopy and Western blotting.ESCC cells were divided into three groups;cells in the interference group and the overexpression group were cultured with exosomes of miR-296-5p in-terference and overexpression cells,respectively;cells in the NC group were cultured with exosomes of NC-transfected cells for 48 h.Cells were collected from each group,cell viability was detected by MTT,and cell migration and invasion were detected by Transwell assay.The expression levels of recombinant cell adhesion molecule-3(CADM3),CADM1,CADM4,E-cadherin,platelet-endothelial cell adhesion molecule-1(CD31)and CD44 were detected by RT-PCR and Western blotting.Results The cell viability,the number of migration cells and the number of invading cells were as fol-lows:the overexpression group>NC group>interference group,and there were significant differences(all P<0.05).The expression levels of CADM1,CADM4 and E-cadherin were as follows:the interference group>NC group>overexpression group,and there were significant differences between groups;the expression levels of CADM3,CD31 and CD44 were high-er in the overexpression group than in the NC group,which were higher than those in the interference group,and the differ-ences were statistically significant(all P<0.05).Conclusion Exosomes with high expression of miR-296-5p may pro-mote the invasion and migration abilities of ESCC cells by regulating the expression levels of CADM,E-cadherin,CD31,CD44,and other genes.
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