Effects of inhibiting expression of hsa_circRNA6448-14 on invasion and migration of human esophageal squamous cell carcinoma cell lines KYSE30 and KYSE150
Objective To investigate the effects of inhibiting the expression of circular RNA(circRNA)hsa_cir-cRNA6448-14 on the invasion and migration of human esophageal squamous cell carcinoma(ESCC)cell lines KYSE30 and KYSE150 and to confirm the biological function of hsa_circRNA6448-14 in ESCC.Methods In vitro passage cul-ture of KYSE30 and KYSE150 cells was conducted.The cells were randomly divided into the KYSE30 knockdown group,KYSE150 knockdown group,KYSE30 control group,and KYSE150 control group,respectively.Cells in the KYSE30 knockdown group and KYSE150 knockdown group were transfected with hsa_circRNA6448-14-siRNA interference plasmid(inhibiting hsa_circRNA6448-14 expression),while cells in the KYSE30 control group and KYSE150 control group were transfected with siNC plasmid(blank plasmid).After 48 h of culture,qRT-PCR was used to detect the expression of hsa_circRNA6448-14 in each group,and we successfully cultured KYSE30 and KYSE150 cells with inhibited hsa_cir-cRNA6448-14 expression.The invasion and migration abilities of cells in each group were detected by Transwell experi-ment and Scratch assay after 24 h of culture.Results The number of transmembrane cells in the KYSE30 knockdown group,KYSE30 control group,KYSE150 knockdown group,and KYSE150 control group after 24 h of culture were 58.00±3.61,161.33±4.06,69.00±2.08,and 191.33±6.39,respectively.Compared with the control groups,the number of transmembrane cells in the knockdown groups was significantly smaller(t=19.04,18.21,respectively;all P<0.05).The migration area of cells in the KYSE30 knockdown group,KYSE30 control group,KYSE150 knockdown group,and KYSE150 control group after 24 h of culture was(11.67±0.88),(26.00±1.73),(14.33±0.88),and(28.00±1.53)mm2,respectively.Compared with the control groups,the knockdown groups showed significantly smaller migration area after 24 h of culture(t=7.37,7.75,respectively;all P<0.05).Conclusions Inhibition of hsa_circRNA6448-14 ex-pression reduces the invasive and migratory abilities of KYSE30 and KYSE150 cells.Hsa_circRNA6448-14 plays a signifi-cant role in the invasion and migration processes of ESCC.
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维普
