Regulatory effects of baicalein on invasion,migration,and EMT in human breast cancer cell line MDA-MB-231
Objective To investigate the regulatory effects of baicalein on the invasion,migration,and epithelial-mesenchymal transition(EMT)of human breast cancer cell line MDA-MB-231 and to explore its potential mechanism of action.Methods MDA-MB-231 cells in the logarithmic growth phase were divided into the Group 1,Group 2,Group 3,and Control group.Baicalein was added to cells in the Group 1,Group 2,and Group 3 at concentrations of 2.5,5,and 10 µmol/L,respectively,while cells in the Control group were not treated.After 48 h of incubation,Scratch repair experi-ment was used to observe cell migration capacity,Transwell invasion experiment was performed to assess cell invasion ca-pacity,and Western blotting was utilized to detect the expression levels of EMT markers including vimentin and E-cad-herin,as well as integrin αv,integrin β3,phosphorylated focal adhesion kinase(p-FAK),and phosphorylated phosphati-dylinositol-3 kinase(p-PI3K)proteins.Results Compared with the control group,the cell migration rate decreased,the number of invading cells decreased,the relative expression level of E-cadherin was higher,and the relative expression levels of vimentin,integrin αv,integrin β3,p-FAK,and p-PI3K protein were lower in the baicalein group,with a dose-de-pendant manner(all P<0.05).Conclusions Baicalein inhibits the invasion,migration,and EMT of MDA-MB-231 cells.Baicalein may inhibit the expression of integrin αv,integrin β3,and further inhibit the expression of p-FAK and p-PI3K proteins,thereby inhibiting the invasion,migration,and EMT of MDA-MB-231 cells.
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