摘要
目的 观察黄岑素对人乳腺癌细胞系MDA-MB-231的侵袭、迁移及上皮间充质转化(EMT)的调控作用,探讨其可能作用机制.方法 取对数生长期MDA-MB-231细胞分为一组、二组、三组及对照组,一组、二组、三组分别加入2.5、5、10 µmol/L的黄岑素,对照组不做任何处理.培养48 h时采用划痕修复实验观察四组细胞迁移能力、采用Transwell侵袭实验观察四组细胞侵袭能力,采用Western Blotting法检测细胞EMT标志物波形蛋白(vimentin)及E-钙黏蛋白(E-cadherin)、整合素αv、β3、磷酸化黏着斑激酶(p-FAK)、磷酸化磷脂酰肌醇3激酶(整合素p-PI3K).结果 与对照组相比,黄岑素组细胞迁移率降低、侵袭细胞数少,细胞E-cadherin相对表达量高,vimentin、整合素αv、整合素β3、p-FAK、p-PI3K蛋白相对表达量低,且呈剂量依赖性(P均<0.05).结论 黄芩素抑制MDA-MB-231细胞的侵袭、迁移及EMT.黄岑素可能通过抑制整合素αv、整合素β3表达,进一步抑制p-FAK、p-PI3K蛋白表达,抑制MDA-MB-231的侵袭、迁移及EMT.
Abstract
Objective To investigate the regulatory effects of baicalein on the invasion,migration,and epithelial-mesenchymal transition(EMT)of human breast cancer cell line MDA-MB-231 and to explore its potential mechanism of action.Methods MDA-MB-231 cells in the logarithmic growth phase were divided into the Group 1,Group 2,Group 3,and Control group.Baicalein was added to cells in the Group 1,Group 2,and Group 3 at concentrations of 2.5,5,and 10 µmol/L,respectively,while cells in the Control group were not treated.After 48 h of incubation,Scratch repair experi-ment was used to observe cell migration capacity,Transwell invasion experiment was performed to assess cell invasion ca-pacity,and Western blotting was utilized to detect the expression levels of EMT markers including vimentin and E-cad-herin,as well as integrin αv,integrin β3,phosphorylated focal adhesion kinase(p-FAK),and phosphorylated phosphati-dylinositol-3 kinase(p-PI3K)proteins.Results Compared with the control group,the cell migration rate decreased,the number of invading cells decreased,the relative expression level of E-cadherin was higher,and the relative expression levels of vimentin,integrin αv,integrin β3,p-FAK,and p-PI3K protein were lower in the baicalein group,with a dose-de-pendant manner(all P<0.05).Conclusions Baicalein inhibits the invasion,migration,and EMT of MDA-MB-231 cells.Baicalein may inhibit the expression of integrin αv,integrin β3,and further inhibit the expression of p-FAK and p-PI3K proteins,thereby inhibiting the invasion,migration,and EMT of MDA-MB-231 cells.
基金项目
黔南民族医学高等专科学校校基金(qnyz202013)
黔南民族医学高等专科学校校基金(qnyz202206)
黔南民族医学高等专科学教育教学科研基金项目(qnyzjx202205)
黔南民族医学高等专科学校大学生科技创新项目(qnyz202201)
维普
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