MTHFR C677T gene polymorphism in patients with H-type hypertension combined with acute ischaemic stroke and its correlation with renal function
Objective To observe the gene polymorphism of methylenetetrahydrofolate reductase(MTHFR)C677T in patients with H-type hypertension combined with acute ischaemic stroke and to analyze its correlation with renal function in these patients.Methods Totally 153 patients with H-type hypertension combined with acute ischaemic stroke were se-lected as the observation group,and 158 patients with non-H-type hypertension combined with acute ischaemic stroke in the same period were selected as the control group.Peripheral venous blood was collected from both groups,and MTHFR C677T genotype was detected by PCR amplification and microarray technology to measure systemic immune inflammation index(SII),and serum creatinine levels were measured using a Hitachi 7600 automatic biochemical analyzer in order to calculate the estimated glomerular filtration rate(eGFR)in both group.Multiple linear regression analysis was employed to assess the correlations of MTHFR C677T genotypes with homocysteine(Hcy)level and eGFR in patients with H-type hy-pertension combined with acute ischaemic stroke,and Spearman correlation analysis was employed to assess the correla-tions of SII with eGFR and Hcy levels in patients with H-type hypertension combined with acute ischaemic stroke.Results Compared with the control group,patients in the observation group had the highest frequency of TT genotype dis-tribution and the highest frequency of T allele(χ2=19.188,5.138,respectively;both P<0.05).The SII of patients in the observation and control groups were 583.54(384.97,903.73)and 425.03(310.26,583.16),respectively,with statis-tically significant difference between these two groups(P<0.05).TT genotype patients with H-type hypertension com-bined with acute ischaemic stroke had higher serum Hcy level and lower eGFR level than patients with CC and CT geno-types(F=28.544,3.749,respectively;both P<0.05).The TT type of MTHFR C677T gene was positively correlated with serum Hcy level in patients with H-type hypertension combined with acute ischaemic stroke(β=4.173,P<0.05)and was negatively correlated with eGFR level(β=-6.559,P<0.05).The SII was positively correlated with serum Hcy level(r =0.226,P<0.05)and was negatively correlated with eGFR level(r=-0.129,P<0.05)in patients with H-type hyperten-sion combined with acute ischaemic stroke.Conclusions Patients with H-type hypertension combined with acute isch-aemic stroke predominantly exhibit the TT genotype of the MTHFR C677T gene.It is suggested that patients with H-type hypertension combined with acute ischaemic stroke carrying the TT genotype of MTHFR C677T may be more prone to expe-riencing a decline in renal function.
万方数据
维普
