Objective Based on the Gene Expression Omnibus(GEO)database,our aim is to identify potential mi-tophagy-related genes for sarcopenia and to observe their expression changes in skeletal muscle tissues of patients with sarco-penia.Methods Gene expression datasets for sarcopenia(GSE1428)were obtained from the GEO database and differen-tially expressed genes(DEGs)in the sarcopenia samples and normal samples were screened.The DEGs of sarcopenia were intersected with mitophagy-related genes using the"VennDiagram"package to obtain mitophagy-related DEGs of sarcope-nia.The biological functions of mitophagy-related DEGs for sarcopenia were analyzed using Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG).The mitophagy-related target genes for sarcopenia were selected using Cyto-scape,and the expression changes of these genes in skeletal muscle tissues of patients with sarcopenia and controls were ob-served in the gene expression datasets GSE136344 for sarcopenia.Results Ninety-nine mitophagy-DEGs for sarcopenia were screened out.As shown by KEGG enrichment analyses,these genes were significantly enriched in pathways responsi-ble for neurodegeneration-multiple diseases,Parkinson's disease,prion disease,and so on.GO function annotation showed that DEGs were mostly located in the mitochondrial inner membrane and mitochondrial protein-containing complex,where they were engaged in biological processes such as energy metabolism,cellular respiration,and oxidative phosphorylation.They were also involved in the molecular functions of active transmembrane transporter activity,etc.The inner membrane mitochondrial protein(IMMT),dynamin 1-like(DNM1L),and ATP5A1 were identified as the mitophagy-related target genes for sarcopenia.Sarcopenic patients exhibited lower expression levels of IMMT and DNM1L in their skeletal muscles in comparison with non-sarcopenic individuals(all P<0.05).Conclusions IMMT and DNM1L were identified as signifi-cant mitophagy-related target genes for sarcopenia.These target genes contributed to the pathogenesis of Sarcopenia not on-ly by affecting neurodegeneration-multiple diseases pathways,Parkinson's disease pathway,and prion disease pathway but also by participating in biological processes such as energy metabolism,cellular respiration,and oxidative phosphorylation.Patients with sarcopenia exhibited lower expression levels of IMMT and DNM1L in skeletal muscle tissues.
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