Interactive protein and mode of AGPS in glioma cells
Objective To explore the interactive protein and mode of oncogene alkylglycerone phosphate synthase(AGPS)in glioma U251 cells.Methods U251 cells were routinely cultured and divided into the control group,shR-AGPS-1 group and shR-AGPS-2 group,respectively,which were added with the negative control lentivirus and AGPS shR-NA lentiviruse with different silencing levels.Western blotting was used to detect the expression of AGPS protein to verify the silencing efficiency.The interactive protein of AGPS was identified by immunoprecipitation and mass spectrometry,and was identified by Western blotting.The homologous modeling of the interactive proteins of AGPS was established by computer simulation and the interaction mode was predicted.Results Compared with the control group,the expression of AGPS decreased in the shR-AGPS-1 group and shR-AGPS-2 group and the expression of AGPS was lower in the shR-AGPS-1 than in the shR-AGPS-2 group(all P<0.05).It was predicted that heterogeneous nuclear ribonucleoprotein K(HNRNPK)was the target protein of AGPS,and both AGPS and HNRNPK were detected in the AGPS immunoprecipita-tion lysate,which indicated that AGPS and HNRNPK formed complexes,and both of them were expressed in the nucleus.The results of computer simulation showed that AGPS and HNRNPK were interacted through amino acid residues based on hydrogen bond,conjugation,hydrophobic bond and electrostatic force.Conclusion The HNRNPK is the interactive protein of AGPS in glioma,and the interaction of hydrogen bond,conjugation,hydrophobic bond and electrostatic force may be the main modes of action of AGPS.
万方数据
维普
