Differential expression genes and functional analysis in visual cortex of amblyopic rats with monocular form deprivation during the critical period of visual development
Objective To screen the differentially expressed genes in the visual cortex of amblyopic rats with monoc-ular form deprivation during the critical period of visual development and to analyze their biological functions.Methods Twenty-four 13-day-old SD rats with their eyes not yet open were randomly divided into the control group and model group,with 12 rats in each.In the model group,right eyelid suture was performed to establish monocular form deprivation amblyo-pia model.At 60 days after birth,the rats were anesthetized and sacrificed,and their brain tissues were collected.Gene chip technology was used to screen the differentially expressed genes.Gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)were used for enrichment analysis of the differentially expressed genes.Results Com-pared with the blank control group,163 differentially expressed genes in the left visual cortex of the model group,38 differ-entially expressed genes in the right visual cortex,and 16 differentially expressed genes in both sides.GO enrichment anal-ysis showed that 22 GO entries were involved in the differentially expressed genes in the left visual cortex with a degree greater than 2,while 19 GO entries were involved in the differentially expressed genes in the right visual cortex.KEGG en-richment analysis showed that the main functions of differentially expressed genes in the model group were involved in the formation of embryonic dorsal ventral axis,light signaling pathway,MAPK signaling pathway,NOD-like receptor signal-ing pathway,neurotrophic protein signaling pathway,neural ligand-receptor interaction signaling pathway,and so on.The abnormal expression of MAPK1 and GNAT2 genes might be related to abnormal visual function.The main functions of MAPK1 gene were involved in the formation of embryonic dorsal ventral axis,MAPK signaling pathway,NOD-like recep-tor signaling pathway,neurotrophic protein signaling pathway,and neural ligand-receptor interaction signaling pathway,whereas GNAT2 was primarily associated with the phototransduction pathway.Conclusions Monocular form deprivation during the critical period of visual development can cause abnormal gene expression in the visual cortex of rats,further lead to changes in the expression of genes in the related signal pathways,leading to abnormal function of visual signal trans-duction.The abnormal expression of MAPK1 and GNAT2 genes may be two critical biological mechanisms underlying the pathogenesis of amblyopia.
amblyopiaform deprivationcritical period of visual developmentgene chip technologyMAPK1 geneGNAT2 gene
万方数据
维普
