Expression of inflammatory response-related genes in different adipose tissues of mice with high-fat diet-induced obesity and its mechanism
Objective To explore the inflammatory response-related genes of different adipose tissues in mice with high-fat diet-induced obesity and its mechanism.Methods Ten mice were randomly divided into the normal diet control group(ND group)and high fat diet model group(HFD group),with 5 mice in each,which were fed with normal diet and high fat diet,respectively,for 10 weeks.The mice were killed by neck dislocation,and the interscapular brown adipose tissue(BAT),inguinal white adipose tissue(iWAT)and epididymal white adipose tissue(eWAT)were removed.The ad-ipose tissue was sequenced by transcriptome sequencing(seq),and the sequencing results were evaluated as well as prin-cipal component analysis(PCA).The differentially expressed genes in three kinds of adipose tissues were screened and an-alyzed by Gene Ontology(GO)functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis,and the first 20 inflammatory related genes with the most up-regulated expression were screened.Results The level of body mass in the HFD group was significantly higher than that in the ND group.The re-sults of seq quality evaluation of adipose tissue in both groups showed that the error rate was<0.1%,with Q20>85%,and Q30>80%.PCA showed that the variance contribution rates of the two principal components were 51.81%and 19.08%,respectively.There were 2 548 up-regulated genes in iWAT of the HFD group,3 384 up-regulated genes in eWAT of the HFD group,1 630 up-regulated genes in BAT of the HFD group,2 082 down-regulated genes in iWAT of the HFD group,2 780 down-regulated genes in eWAT of the HFD group,and 1 128 down-regulated genes in BAT of the HFD group.Among the three kinds of up-regulated genes in the adipose tissues,they were mainly enriched in the processes of inflammation and immune response,while in all the down-regulated genes in three kinds of adipose tissues,they were mainly enriched in the processes of lipid metabolism and small molecule metabolism.In the HFD group,the main enrich-ment pathways were classical inflammatory signal pathways NF-kappa B,PI3k-Akt and Toll-like receptor.Conclusion There are differentially expressed genes related to inflammation in the three kinds of adipose tissues of obese mice induced by high-fat diet,and there are different response mechanisms for inflammation.
万方数据
维普
