Effects of anlotinib regulating NF-κB signaling pathway on proliferation and apoptosis of glioma cells
Objective To investigate the effects of anlotinib on proliferation and apoptosis of human glioma cells(T98G cells)through nuclear transcription factor-κB(NF-κB)signaling pathway.Methods T98G cells were cultured in vitro and randomly divided into the control group,5 µmol/L anlotinib group,10 µmol/L anlotinib group,20 µmol/L anlotinib group,positive drug group,and inhibitor group.Cells in the control group were not intervened,cells in the 5,10,and 20 µmol/L anlotinib group were treated with 5,10 and 20 µmol/L anlotinib,respectively,and cells in the posi-tive drug group were treated with 50 mg/L 5-fluorouracil;in the inhibitor group,10 µmol/L anlotinib +5 µmol/L NF-κB pathway inhibitor BAY 11-7082 were added for intervention.Cell viability was measured by CCK-8,cell proliferation rate was measured by 5-acetylidene-2'deoxyuracil riboside method,and apoptosis rate was measured by Hoechst33258 stain-ing.The expression levels of cell cycle proteins D1(CyclinD1),Caspase-3,NF-κB p65 and phosphorylated(p-)NF-κB p65 were detected by Western blotting.Results Compared with the control group,there was no significant difference in cell viability in the 5 µmol/L anlotinib group(P>0.05),while the cell viability in the 10,20 µmol/L anlotinib groups and positive drug group was lower(all P<0.05).Compared with the control group,the cell proliferation rates,and the ex-pression levels of CyclinD1 and p-NF-κB p65 protein were lower,while the apoptosis rates and the expression levels of Cas-pase-3 protein were higher in each anlotinib group and positive drug group(all P<0.05).Compared with the positive drug group,the cell proliferation rate,the expression levels of CyclinD1 and P-NF-κB p65 protein in the 10 µmol/L anrotinib group were higher(all P<0.05),while the apoptosis rate and the expression of Caspase-3 protein were lower(both P<0.05).There was no significant difference between 20 µmol/L anlotinib group and positive drug group(P>0.05).Com-pared with the 10 µmol/L anrotinib group,the changes of all indexes in the inhibitor group were more significant(all P<0.05).Conclusion Anlotinib may inhibit proliferation and promote apoptosis of human glioma cells by inhibiting NF-κB pathway signaling.
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