Effects of dexmedetomidine on IL-1β-induced apoptosis and inflammatory response of human nucleus pulposus cells
Objective To observe the effects of dexmedetomidine(Dex)on apoptosis and inflammatory response of human nucleus pulposus cells induced by interleukin-1β(IL-1β).Methods Human nucleus pulpocytes were cultured in vitro and divided into the Control group(without any treatment),IL-1β group(cultured with 10 ng/mL IL-1β for 24 h),IL-1β+Dex-L group(cultured with 25 nmol/L dexmedetomidine and 10 ng/mL IL-1β for 24 h),IL-1β+Dex-M group(cultured with 50 nmol/L dexmedetomidine and 10 ng/mL IL-1β for 24 h),and IL-1β+Dex-H group(cultured with 100 nmol/L dex-medetomidine and 10 ng/mL IL-1β for 24 h),respectively.The cell survival rate was measured by MTT,the apoptosis rate was detected by flow cytometry,and the tumor necrosis factor-α(TNF-α),interleukin-8(IL-8),nitric oxide(NO)and in-terleukin-6(IL-6)were detected by enzyme-linked immunosorbent assay(ELISA).The expression levels of B-cell lympho-ma/leukemia 2(Bcl-2),Bcl-2 associated X protein(Bax),inducible-nitric oxide synthase(iNOS)and cyclooxygenase-2(COX-2)were detected by Western blotting.Results Compared with the Control group,the cell survival rates at 24 h and 48 h and Bax protein expression in the IL-1β group decreased,while the apoptosis rate,Bax protein expression,TNF-α,IL-6,IL-8,COX-2,iNOS protein expression and NO expression increased,with statistically significant difference(all P<0.05).Compared with the IL-1β group,the survival rates of cells at 24 h and 48 h were higher,and the expression of Bcl-2 protein was higher,the apoptotic rate,the expression levels of Bax protein,TNF-α,IL-6,IL-8,COX-2,iNOS protein,and NO were lower in the IL-1β+Dex-L group,IL-1β+Dex-M group,and IL-1β+Dex-H group,and the differences were all statistically significant(all P<0.05).The survival rates of cells at 24 h and 48 h and the expression of Bcl-2 protein in-creased successively,the apoptotic rate,the expression levels of Bax protein,TNF-α,IL-6,IL-8,COX-2,iNOS protein,and NO decreased successively in the IL-1β+Dex-L group,IL-1β+Dex-M group,and IL-1β+Dex-H group,and the differ-ences were all statistically significant(all P<0.05).Conclusion Dexmedetomidine can inhibit the apoptosis of human nucleus pulposus cells induced by IL-1β and reduce the inflammatory response in a concentration-dependent manner.
degeneration of lumbar intervertebral discdexmedetomidineinterleukin-1βnucleus pulposus cellsapoptosisinflammatory response
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维普
