Alterations in expression of LLT1 in liver tissues of patients with alveolar echinococcosis
Objective To observe the alterations in lectin-like transcript 1(LLT1)expression within liver tissues of alveolar echinococcosis(AE)patients and to assess its correlation with clinical pathological parameters.Methods Thir-ty liver tissue samples from AE patients were collected,including 30 samples from proximal lesion tissues(CLT,<2 cm form the lesion center)and 28 samples from distal tissues(DLT,>2 cm from the lesion center).Immunohistochemistry was employed to determine the expression and localization of LLT1.The LLT1 expression changes in the liver tissues of AE patient with different clinical pathological parameters were analyzed.Data set GSE124362 from the GEO database,includ-ing 6 CTL and 6 matched DLT samples,was used to analyze the correlation between CLEC2D(the LLT1 coding gene)ex-pression and immune cell expression.Results LLT1 predominantly expressed in hepatocytes and inflammatory cells within the lesions'surrounding inflammatory zone.The expression scores for LLT1 in CLT and DLT were 3(2,4)and 6(4,8),respectively,indicating significantly higher expression levels in CLT(both P<0.05).LLT1 expression was relat-ed to the AE patients'P stage,ALT,AST,and γ-glutamyl transferase levels(all P<0.05).CLEC2D expression showed a positive correlation with plasma cells,M2 macrophages,and activated dendritic cells(r=0.83,0.87,0.74,respective-ly;all P<0.01),and had a negative correlation with activated NK cells,M1 macrophages,and mast cells(r=-0.73,-0.60,-0.74,respectively;all P<0.01).Further analysis revealed that CLEC2D expression was negatively correlated with M1 mac-rophage markers CD1B and IL-6(r=-0.65,-0.89,respectively;both P<0.05),and was positively correlated with M2 macrophage markers CD163,CLEC7A,and IL-10(r=0.90,0.71,0.74,respectively;all P<0.01).Conclusion The high expression of LLT1 in CLT of AE patients was related to P stage,liver function indices,and various immune cell ex-pression,suggesting LLT1 was involved in infiltration process of the AE liver lesions.
万方数据
维普
