Analysis on mechanism of action of Jinyinhua-Daxueteng in treatment of rheumatoid arthritis
Objective To analyze the mechanism of Jinyinhua-Daxueteng in the treatment of rheumatoid arthritis(RA).Methods The drug effects of Jinyinhua-Daxueteng and the disease targets of RA were obtained through databases such as TCMSP,Genecards,OMIM,TTD,etc.The intersection of drug targets and disease targets were searched based on the Venny platform.A"drug-component-key target-disease"network was established using Cytoscape software and VLOOKUP function.The protein-protein interaction network for key targets was constructed using String database.GO analysis and KEGG analysis were used on key targets.Core ingredients and core targets were used for molecular docking.Results A total of 323 targets were obtained from Jinyinhua-Daxueteng,and 5 093 targets for RA disease were obtained.PTGS1 and PTGS2 core targets were screened from the"drug-component-key target-disease"network,including eugenol,emodin,β-Sitosterol,ursolic acid,kaempferol,luteolin,trihydroxyflavones and quercetin.Jinyinhua-Daxueteng mainly participated in the regulation of cellular response to nitrogen compounds,lymphocyte differentiation,growth factor regula-tion,LPS induced inflammatory response,through the AGE-RAGE signaling pathway,synovial cell apoptosis signaling pathway,HIF-1 signaling pathway,NF-κB signaling pathway,JAK-STAT signaling pathway,and RAS signaling path-way.The molecular docking results showed good affinity between the 8 core components and the 2 core targets.Conclusion Jinyinhua-Daxueteng may participate in alleviating the inflammatory response caused by autoimmune disorders in RA pa-tients by regulating the expression of PTGS1 and PTGS2.
万方数据
维普
