Effect of central cholinergic neuron degeneration on postoperative cognitive function in mice after hepatolobectomy
Objective To explore the effect of central cholinergic neuron degeneration on postoperative cognitive function in mice after hepatolobectomy.Methods C57BL/6 mice were randomly divided into the anesthesia group,sur-gery group,donepezil group,mu-p75-sap group,and control group,with 5 mice in each group.The animals in the anes-thesia group were anesthetized by inhaling a mixture of 2.6%sevoflurane,30%oxygen,and 70%nitrogen for 6 h.Mice in the surgery group,donepezil group,and mu-p75-sap group underwent hepatic lobectomy under the same anesthesia meth-od,with the donepezil group receiving selective reversible central acetylcholinesterase(AChE)inhibitor donepezil 5 mg/kg for 4 weeks before surgery,and the mu-p75-sap group receiving bilateral intraventricular injection of immunotoxin mu-p75-sap 0.8 µg per side to create a model of cholinergic neuron degeneration after anesthesia.Mice in the control group did not undergo any surgery or anesthesia procedures.The Morris water maze experiment was used to assess the learning and memory abilities of the mice,and the Cortez Bright Lanthanide protein assay kit was used to detect central cholinergic neurobiomarkers[choline acetyltransferase(ChAT),AChE,and acetylcholine(Ach)]in the hippocampal tissues of each group.Results Compared with the control group,the surgery group showed a prolonged escape latency,increased AChE expression,decreased retention time,platform crossings,and reduced ChAT and Ach expression in the hippocam-pal tissues(all P<0.05).Compared with the surgery group,the donepezil group exhibited a shortened escape latency,in-creased retention time,platform crossings,decreased AChE expression,and increased ChAT and Ach expression;the mice in the mu-p75-sap group showed a prolonged escape latency,decreased retention time and platform crossings,de-creased ChAT and Ach expression,and increased AChE expression in comparison with those of the surgery group(all P<0.05).Conclusions Hepatic lobectomy can affect postoperative cognitive function in mice.Central cholinergic neuron degeneration may exacerbate the impact on postoperative cognitive function,potentially leading to the development and pro-gression of postoperative cognitive impairment.
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