Screening of differentially expressed genes in cerebellar tissues of TMEM206-knockout mice and analysis of their biological functions
Objective To screen the differentially expressed genes in the cerebellar tissues of TMEM206-knockout mice and to analyze their biological functions to explore the function of the TMEM206 gene.Methods TMEM206-knock-out mice were generated via the CRISPR-Cas9 system.The homozygous TMEM206-knockout mice were obtained by mat-ing.Total RNAs were extracted from cerebellum in three 30-week-old homozygous TMEM206-knockout female mice and three wild-type female littermates.Transcriptome sequencing was performed in cerebellum,the differentially expressed genes were screened using Cuffdiff(v2.2.1)software with EBSeq algorithm.GO functional enrichment analysis and KEGG pathway analysis were performed using the DAVID database.Results Totally 474 differentially expressed genes were screened out,including 265 up-regulated genes and 209 down-regulated genes in the cerebellar tissues of TMEM206-knockout mice.Gene ontology analysis showed that these differentially expressed genes were mainly focused on the exter-nal side of plasma membrane and major histocompatibility complex I;the molecular function involved in sequence-specific DNA binding transcription factor activity,lipid binding,and antigen binding.Differentially expressed genes were involved in many biological processes,such as cell response to DNA damage,cell differentiation selection,bone mineralization,etc.KEGG pathway enrichment analysis showed that the differential expression genes were mainly cell adhesion molecules and tumor necrosis factor pathway molecules in terms of molecular properties,and were mainly related to cytophagocytosis and senescence in function,and were related to the infection of human papillomavirus,human T-lymphocytic leukemia vi-rus type I,herpes simplex virus,human herpesvirus type ⅤⅢ and EB virus.Conclusion After TMEM206 gene knock-out,474 differentially expressed genes are screened out in the mouse cerebellar tissues,and these genes are related to the stability of extracellular membrane structure,cell adhesion and virus infection.
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