Relationships between serum miR-223,IL-6 and brain damage in children with complex febrile convulsions
Objective To investigate the relationships between serum microRNA-223(miR-223),interleukin-6(IL-6)and brain damage in children with complex febrile seizures(CFS).Methods Totally 124 children with CFS(CFS group)were divided into the brain injury group of 39 cases and the non-brain injury group of 85 cases according to whether they had brain injury or not;meanwhile,46 healthy children at the same period(control group)were selected.Real-time fluorescence quantitative polymerase chain reaction and enzyme-linked immunosorbent assay were used to detect serum miR-223 and IL-6 expression.Spearman's correlation was used to analyze the correlation of serum miR-223 and IL-6 levels in the serum of CFS patients,multifactorial Logistic regression was used to analyze the factors affecting brain inju-ry in children with CFS,and the predictive value of serum miR-223 and IL-6 levels in children with CFS was analyzed by using receiver operating characteristic(ROC)curve.Results Compared with the control group,serum miR-223 level decreased and IL-6 level increased in the CFS group(both P<0.05).Serum miR-223 was negatively correlated with IL-6 level in children with CFS(r=-0.800,P<0.05).The incidence of brain injury in 124 children with CFS was 31.45%(39/124).Generalized convulsions,the number of convulsive seizures≥2,duration of convulsive seizures≥5 min,and elevated IL-6 were independent risk factors for brain injury in children with CFS,and elevated miR-223 was an indepen-dent protective factor(all P<0.05).The area under the curve of serum miR-223 combined with IL-6 level in prediction of brain injury of children with CFS was 0.899,which was greater than that(0.803,0.780)of serum miR-223 and IL-6 lev-els alone(both P<0.05).Conclusions Decreased serum miR-223 level and increased IL-6 level are closely associated with brain injury in children with CFS,and the combination of serum miR-223 and IL-6 levels is of high value in predicting brain injury in children with CFS.
万方数据
维普
