Effect of lncR-FOXD2-AS1 regulating miR-145-5p on biological characteristics of non-small-cell lung cancer cells
Objective To investigate the effects of long non-coding RNA(lncR)-FOXD2-AS1 regulating microRNA(miR)-145-5p on proliferation,migration,invasion,and epithelial-mesenchymal transition(EMT)in non-small-cell lung cancer(NSCLC)cells.Methods Human NSCLC cell lines A549,H1299,SK-MES-1,and human normal pulmonary bronchial epithelial cell line 16HBE were cultured in vitro.The expression of lncR-FOXD2-AS1 and miR-145-5p in each cell line was detected using RT-qPCR.SK-MES-1 cells with relatively high expression of lncR-FOXD2-AS1 were selected as the research object for knockdown experiments.SK-MES-1 cells were divided into the NC group(transfected with si-NC)and si-FOXD2-AS1 group(transfected with si-FOXD2-AS1)according to different transfection treatments.After transfection,qRT-PCR was used to detect the expression of lncR-FOXD2-AS1 and miR-145-5p in the cells,CCK-8 was used to detect cell proliferation activity,Transwell experiment was used to detect cell migration and invasion abilities,and Western blotting was used to detect the expression of E-cadherin,N-cadherin,and Fibronectin proteins in cells.The dual luciferase experiment verified the relationship between lncR-FOXD2-AS1 and miR-145-5p.Results Compared with 16HBE cells,lncR-FOXD2-AS1 was highly expressed in NSCLC cell lines(P<0.05),and miR-145-5p was low expressed in NSCLC cell lines(P<0.05).Compared with the NC group,the expression of miR-145-5p in the si-FOXD2-AS1 group increased(P<0.05),the OD values at 0,24,and 48 h decreased(all P<0.05),the number of migrating and invading transmembrane cells became smaller(P<0.05),the expression of E-cadherin protein increased(P<0.05),and the ex-pression levels of N-cadherin and Fibronectin proteins decreased(all P<0.05).The dual luciferase experiment confirmed that miR-145-5p was the target gene of lncR-FOXD2-AS1(P<0.05).Conclusions The expression of lncR-FOXD2-AS1 is elevated in NSCLC.Knocking down lncR-FOXD2-AS1 inhibits the proliferation,migration,invasion,and EMT processes of NSCLC cells by targeting miR-145-5p.
万方数据
维普
