Effects of hepatic ischemia-reperfusion on blood-brain barrier permeability and brain tissue apoptosis in young mice
Objective To observe the effects of hepatic ischemia-reperfusion(HIR)on blood-brain barrier(BBB)permeability and brain tissue apoptosis in young mice.Methods Twenty-four healthy and clean grade C57BL/6 young mice were randomly divided into the sham operation group,dextran glycoside 10 group,and dextran glycoside 40 group,with 8 mice in each group.The HIR models were established by clamping the left hepatic artery and portal vein in mice of both dextran glycoside 10 and 40 groups.After 60 min of reperfusion,dextran glycoside with a molecular weight of 10 and 40 Kd was injected into the inferior vena cava,respectively.Mice in the sham operation group only underwent abdominal opening and closing,free blood vessels,and other procedures.Immunofluorescence staining was used to observe the per-meability of brain tissue BBB(expressed as the pass rate of dextran glycoside),Western blotting was used to detect the in-dicators related to BBB tight junction protein in brain tissues,including Claudin,Occludin and β-Catenin and tight junc-tion protein 1(ZO-1),and TUNEL method was used to detect the apoptosis rate of brain tissues.Results Compared with the sham operation group,the dextran glycoside 10 group and the dextran glycoside 40 group had higher passing rates of dextran glycoside and apoptosis rates in the brain tissues of young mice,and the low-dose group had a higher passing rate of dextran glycoside(all P<0.05).Compared with the sham operation group,the relative expression levels of Clau-din,Occludin,and β-Catenin and ZO-1 proteins decreased(all P<0.05),and there were no statistically significant differ-ences in the above indicators between the dextran glycoside 10 and 40 groups(all P>0.05).Conclusion HIR can lead to increased BBB permeability and increased apoptosis of brain tissues in young mice.
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