Effect of FLIPU combined with local injection of PRP in repairing facial nerve injury in rats
Objective To compare the repair effects of focused low-intensity pulse ultrasound(FLIPUS)combined with local injection of platelet-rich plasma(PRP)on facial nerve injury in rats.Methods Twenty healthy male SD rats were randomly divided into the model group,PRP group,FLIPUS group,and PRP+FLIPUS group,with 5 rats in each group.We established the right lateral facial nerve injury models in each group,and rats in the PRP group received local injection of 50 µL PRP at the nerve injury site,twice a week;rats in the FLIPUS group received FLIPUS treatment at the site of nerve injury for 10 min,once per day;rats in the PRP+FLIPUS group were treated with local injection of PRP and FLIPUS according to the above method;rats in the model group were not subject to special treatment;and the treatment pe-riod for each group was 28 d.Behavioral assessments were conducted weekly after surgery in each group,and scores of beard movement and blink reflex were recorded.Before and on the 28th day after surgery,right lateral nerve electrophysio-logical examination was performed using an electromyography evoked potential device in each group.The maximum CMAP amplitude of the injured facial nerve was recorded and the R value was calculated.After rats of each group were eutha-nized,the injured facial nerve segment tissues were taken and stained with HE for histopathological observation.At the same time,the average number,diameter,and area of axons per unit area were recorded.Results There were no signifi-cant differences in the blink reflex or beard movement scores in rats at 1-4 weeks after surgery between groups(all P>0.05).Compared with the same group before surgery,the maximum CMAP amplitude of facial nerve injury in rats of each group decreased after surgery(P<0.05),and there were no significant differences among all groups after operation(all P>0.05).Compared with the model group,the R values of the injured facial nerves in the PRP group,FLIPUS group,and PRP+FLIPUS group all increased(all P<0.05),while there was no statistically significant difference between the PRP group,FLIPUS group,and PRP+FLIPUS group(all P>0.05).Compared with the model group,the PRP group,FLIPUS group,and PRP+FLIPUS group showed reduced degree of nerve bundle degeneration,demyelination,and axonal vacuol-ization,increased number of regenerated axons,and more orderly arrangement of nerve fibers.Compared with the model group,the number of axons per unit area in the injured facial nerve segment tissue of rats in the PRP group,FLIPUS group,and PRP+FLIPUS group increased(all P<0.05);the axon diameter and area per unit area of the injured facial nerve segment tissue in the PRP group rats were higher than those in the model group,FLIPUS group,and PRP+FLIPUS group(all P<0.05).Conclusion The repair effect of FLIPUS combined with local injection of PRP on facial nerve inju-ry in rats is not superior to that of FLIPU and PRP alone.
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