Effects of isorhynchophylline on inflammation,apoptosis and ERK signaling pathway of acute pancreatitis cells in rats
Objective To observe the effects of isorhynchophylline(LSO)on inflammation,apoptosis and ERK sig-naling pathway of acute pancreatitis(AP)cells in rats.Methods AR42J cells were divided into six groups.Cells in the control group were cultured normally;cells in the model group,LSO group,inhibitor group,LSO+ inhibitor group and LSO+ activator group were added with 100 nmol/L cerulein to prepare AP cell models,and cells in the LSO group were added with 40 μmol/L LSO after modeling,10 μmol/L U0126 was added to the inhibitor group,40 μmol/L LSO and 10 μmol/L U0126 were added to the LSO+ inhibitor group,and 40 μmol/L LSO and 0.1 μmol/L C16-PAF were added to the LSO+ activator group.Inflammatory factors(TNF-α,IL-6,IL-8,IL-1β),proliferation rate,and apoptosis rate in cell cul-ture medium were determined by ELISA,EdU method and Hoechst 33258 staining,respectively.The mRNA levels of nu-cleotide oligomerization domain-like receptor protein 3(NLRP3),speckle-like protein(ASC)and Caspase-1 in the cells were determined by qPCR,and the protein levels of ERK 1/2,p-ERK 1/2,NLRP3,ASC and Caspase-1 were determined by WB.Results Compared with the control group,TNF-α,IL-6,IL-8,IL-1β,apoptosis rate,p-ERK 1/2 protein,NL-RP3,ASC,Caspase-1 mRNA and protein increased,and the proliferation rate decreased in the model group(all P<0.05).Compared with the model group,inflammatory cytokines(TNF-α,IL-6,IL-8,IL-1β),apoptosis rate,p-ERK 1/2 protein,NLRP3,ASC,Caspase-1 mRNA and protein decreased,and proliferation rate increased in the LSO group and inhibitor group(all P<0.05).Compared with the LSO group,U0126 in the LSO+ inhibitor group enhanced the effect of LSO on the above trend of cells,while C16-PAF in the LSO+ activator group reversed the effect of LSO on the above trend of cells(all P<0.05).Conclusion LSO can inhibit the inflammation and apoptosis of AP cells probably by regulating ERK signaling pathway.
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