Screening and biological function analysis of differentially expressed genes in esophageal mucosa of rats with iron accumulation
Objective To screen the differentially expressed genes(DEGs)in the esophageal mucosa of rats with iron accumulation and to analyze the biological function of DEGs.Methods Twelve 6-week-old male Sprague-Dawley rats were divided into the iron accumulation group(n=6)and control group(n=6).Rats in the iron accumulation group re-ceived bi-daily injections of sucrose iron solution,while rats in the control group received the equal volume of normal sa-line.After 14 weeks,the blood,esophagus and other organs were collected,and histological and serological techniques were employed to confirm weather modeling was successful or not.Esophageal mucosa was dissected for transcriptome se-quencing,and DEGs were screened and annotated.The functional annotations of DEGs were conducted by Gene Ontology(GO)and the classification annotations of DEGs were conducted by clusters of orthologous groups for eukaryotic complete genomes(KOG).Results A total of 9 DEGs were screened;5 significantly up-regulated genes included circadian-asso-ciated repressor of transcription(Ciart),vacuolar protein sorting 25 homolog(Vps25),thyroid hormone-responsive pro-tein(Thrsp),UDP-N-acetylglucosamine pyrophosphorylase 1(Uap1),and ADAM metallopeptidase Domain 33(Ad-am33);4 significantly down-regulated genes included peroxidasin(Pxdn),heparan sulfate proteoglycan 2(Hspg2),cen-trosomal protein 2(Cep2)and regulator of G-protein signaling 4(Rgs4).GO enrichment analysis showed that the biologi-cal process(BP)of up-regulated genes was mainly concentrated in rhythmic process,metabolic process,behavior,regula-tion of biological process,establishment of localization,negative regulation of biological process,biological regulation,lo-calization,cellular process,and multicellular biological process;the cellular component(CC)was mainly concentrated in membrane-enclosed lumen,organelle part,organelle,membrane,extracellular region part,membrane part,cell part,cell;the molecular function(MF)was mainly concentrated in binding,structural molecular activity,and catalytic activi-ty;the BP of down-regulated genes was mainly concentrated in detoxification,response to stimulus,cellular component or-ganization or biogenesis,signaling,negative regulation of biological process,positive regulation of biological process,de-velopmental process,cellular process,regulation of biological process,biological regulation,and metabolic process;the CC was mainly concentrated in extracellular matrix,extracellular matrix component,extracellular region part,extracellu-lar region,organelle,membrane,cell part,and cell;the MF was mainly concentrated in enzyme regulation activity,anti-oxidant activity,molecular function regulator,receptor regulator activity,structural molecular activity,binding,and cata-lytic activity.KOG enrichment analysis showed that 3 of the up-regulated genes were annotated,Uap1 was annotated to cell wall/membrane/envelope biogenesis,Adam33 was annotated to posttranslational modification,protein turnover,chap-erones,and Vps25 was annotated to general function prediction only,while 2 of the down-regulated genes were annotated,Rgs4 was annotated to signal transduction mechanisms,and Hspg2 was annotated to posttranslational modification,protein turnover,and chaperone.Conclusions A total of 9 DEGs were screened out,including five significantly up-regulated genes and four significantly down-regulated genes.GO enrichment showed that the BP of DEGs included rhythmic pro-cess,metabolic processes,cellular process,and regulation of biological processes,etc.,CC of DEGs included organelle,membrane,extracellular region,and cell,etc.,MF of DEGS included binding,catalytic activity,and structural molecu-lar activity,etc.KOG enrichment showed that five DEGs were annotated with KOG functions,including posttranslational modification,protein turnover,chaperones,signal transduction and others.These DEGs were mainly involved in the path-ways including cell cycle,cell metabolism,cell proliferation,and oxidative stress,etc.
iron accumulationdifferentially expressed genesgene ontologyclusters of orthologous groups for eu-karyotic complete genomes
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