黄芪皂苷Ⅱ对肾透明细胞癌细胞迁移和侵袭能力的抑制作用及其机制
Inhibitory effects of astragaloside Ⅱ on invasion and migration of clear cell renal cell carcinoma
赵凯 1李勋华 1王科1
作者信息
- 1. 青岛大学附属医院泌尿外科,山东青岛 266003
- 折叠
摘要
目的 探讨黄芪皂苷Ⅱ(ASⅡ)对肾透明细胞癌细胞迁移和侵袭的抑制作用,并分析该作用与Wnt/β-catenin信号通路的关系.方法 取人肾透明细胞癌细胞786-O,随机分为对照组、ASⅡ组和LiCl干预组.对照组仅更换新鲜血清培养基,不进行其他处理;ASⅡ组加入100 μmol/L ASⅡ溶液;LiCl干预组先加入10 mmol/L LiCl(Wnt/β-catenin信号通路激活剂)溶液,24 h后加入100 μmol/L ASⅡ溶液.采用细胞划痕实验观察各组细胞迁移能力,Transwell实验观察细胞侵袭能力,Western blotting法检测细胞上皮间充质转化(EMT)相关蛋白E-钙黏蛋白(E-cadherin)、N-钙黏蛋白(N-cadherin)、波形蛋白(Vimentin).结果 与对照组比较,ASⅡ组细胞迁移率降低;与ASⅡ组比较,LiCl干预组细胞迁移率升高(P均<0.01).与对照组比较,ASⅡ组穿膜细胞数减少;与ASⅡ组比较,LiCl干预组穿膜细胞数增加(P均<0.01).与对照组比较,ASⅡ组E-cadherin蛋白表达水平升高,N-cadherin、Vimentin及β-catenin蛋白表达水平降低;与ASⅡ组比较,LiCl干预组E-cadherin蛋白表达水平降低,N-cadherin、Vimentin及β-catenin蛋白表达水平升高(P均<0.01).结论 ASⅡ能够抑制肾透明细胞癌细胞的迁移和侵袭能力,其机制可能是通过调控Wnt/β-catenin信号通路来抑制细胞EMT实现的.
Abstract
Objective To explore the inhibitory effects of astragaloside Ⅱ(ASⅡ)on invasion and migration of clear cell renal cell carcinoma cells,and to analyze the relationships between the effects and Wnt/β-catenin signaling pathway.Methods Human clear cell renal cell carcinoma cells(786-O)were taken and were randomly divided into three groups:the control group,the ASⅡ group,and the LiCl intervention group.In the control group,only fresh serum was used to re-place the culture medium throughout the experiment without any other treatments;in the ASⅡ group,100 μmol/L ASⅡsolution was added to the cells;in the LiCl intervention group,cells were treated with 10 mmol/L LiCl solution,and after 24 h,they were added with 100 μmol/L ASⅡ solution.The migration ability of the cells in each group was measured by Scratch assay,the invasive ability of the cells was detected by Transwell assay,and epithelial-mesenchymal transition(EMT)-related proteins E-cadherin,N-cadherin,and N-cadherin were detected by Western blotting.Results Com-pared with the control group,the cell migration rate decreased in the ASⅡ group;compared with the ASⅡ group,the cell migration rate increased in the LiCl intervention group(both P<0.01).Compared with the control group,the number of transmembrane cells decreased in the ASⅡ group;compared with the ASⅡ group,the number of transmembrane cells in-creased in the LiCl intervention group(both P<0.01).Compared with the control group,the expression level of E-cad-herin protein increased in the ASⅡ group,while the protein expression levels of N-cadherin,Vimentin and β-catenin de-creased.Compared with the ASⅡ group,the expression level of E-cadherin protein in the LiCl intervention group de-creased,while the protein expression levels of N-cadherin,Vimentin and β-catenin increased(all P<0.01).ConclusionsⅡ can inhibit EMT via regulating Wnt/β-catenin signaling pathway.
关键词
黄芪皂苷Ⅱ/肾肿瘤/Wnt/β-catenin信号通路/细胞迁移/细胞侵袭/上皮间充质转化Key words
astragaloside Ⅱ/kidney neoplasms/Wnt/β-catenin signaling pathway/cell migration/cell inva-sion/epithelial-mesenchymal transition引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据