Inhibitory effects of astragaloside Ⅱ on invasion and migration of clear cell renal cell carcinoma
Objective To explore the inhibitory effects of astragaloside Ⅱ(ASⅡ)on invasion and migration of clear cell renal cell carcinoma cells,and to analyze the relationships between the effects and Wnt/β-catenin signaling pathway.Methods Human clear cell renal cell carcinoma cells(786-O)were taken and were randomly divided into three groups:the control group,the ASⅡ group,and the LiCl intervention group.In the control group,only fresh serum was used to re-place the culture medium throughout the experiment without any other treatments;in the ASⅡ group,100 μmol/L ASⅡsolution was added to the cells;in the LiCl intervention group,cells were treated with 10 mmol/L LiCl solution,and after 24 h,they were added with 100 μmol/L ASⅡ solution.The migration ability of the cells in each group was measured by Scratch assay,the invasive ability of the cells was detected by Transwell assay,and epithelial-mesenchymal transition(EMT)-related proteins E-cadherin,N-cadherin,and N-cadherin were detected by Western blotting.Results Com-pared with the control group,the cell migration rate decreased in the ASⅡ group;compared with the ASⅡ group,the cell migration rate increased in the LiCl intervention group(both P<0.01).Compared with the control group,the number of transmembrane cells decreased in the ASⅡ group;compared with the ASⅡ group,the number of transmembrane cells in-creased in the LiCl intervention group(both P<0.01).Compared with the control group,the expression level of E-cad-herin protein increased in the ASⅡ group,while the protein expression levels of N-cadherin,Vimentin and β-catenin de-creased.Compared with the ASⅡ group,the expression level of E-cadherin protein in the LiCl intervention group de-creased,while the protein expression levels of N-cadherin,Vimentin and β-catenin increased(all P<0.01).ConclusionsⅡ can inhibit EMT via regulating Wnt/β-catenin signaling pathway.
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