Effect of injecting NaHS into left renal artery on renal ischemia-reperfusion injury in rats and its mechanism
Objective To investigate the effect of sodium hydrosulfide(NaHS)on renal ischemia-reperfusion(I/R)injury in rats,and the relationship between its mechanism and Klotho protein.Methods We selected 18 male SD rats and randomly divided them into the sham group,I/R group,and NaHS intervention group(I/R+NaHS group),with 6 rats in each group.In the I/R group,we removed the right kidney,and then used non-invasive arterial clips to clamp the left renal pedicle to simulate the state of acute renal ischemia,and released the arterial clip after 45 minutes of clamping to simulate the state of renal reperfusion.After the acute renal ischemia model was established in the NaHS intervention group,NaHS was injected into the left renal artery,followed by reperfusion.The sham operation group was not clamped.After 24 h of reperfusion,blood samples were collected from each group,and serum renal function indexes such as urea ni-trogen(BUN)and creatinine(Scr)were detected by colorimetry.Renal tissue samples were taken from each group,and the histopathological changes of kidney were observed by HE staining,the content of SOD and MDA in renal tissues were detected by colorimetry,and the expression levels of Klotho and Nrf2 protein in renal tissues were detected by Western blotting.Results Compared with the sham group,serum creatinine and urea nitrogen increased,renal tubular injury was aggravated,SOD activity decreased,MDA content increased,the expression of Klotho protein in the renal tissues de-creased,and the expression of Nrf2 proteion increased in the I/R group(P<0.05 or P<0.01).Compared with the I/R group,the serum levels of creainine and urea nitrogen decreased,renal tubular injury was alleviated,SOD activity in-creased,MDA content decreased,the Klotho protein expression increased,and the Nrf2 protein expression decreased in the I/R+NaHS group(P<0.05 or P<0.01).Conclusion NaHS can alleviate renal I/R injury in rats with acute renal ischemia,and its mechanism may be related to up-regulating Klotho protein expression and reducing oxidative stress.
国家科技期刊平台
NSTL
万方数据
