Effects of miR-138 on proliferation,migration,and invasion of renal cancer cells
Objective To observe the effects of miR-138 on proliferation,migration,and invasion of renal cancer cells,and to explore its targeted regulatory effect on cyclin D1(CCND1).Methods Kidney cancer cells 786-O were taken and randomly divided into the control group,miR-138 overexpression group,and miR-138 low expression group,which were transfected with miR-138 null liposome,miR-138 mimic,and miR-138 inhibitor,respectively.CCK-8 assay was used to observe the proliferation of the cells in each group,and the Scratch assay was used to observe the cell migration ability.Transwell assay was used to observe cell invasion ability.The target genes of miR-138 were predicted by StarBase software.The luciferase assay was utilized to determine the targeted regulatory relationship between miR-138 and CCND1.Results Compared with the control group,miR-138 overexpression group showed decreased cell proliferation activity,decreased distance of cell migration and the number of cell invasion,and decreased CCND1 protein expression(all P<0.01).Compared with the control group,the miR-138 low expression group showed elevated cell proliferative activity,increased distance of cell migration and the number of cell invasion,and elevated CCND1 protein expression(all P<0.01).StarBase software predictive result indicated that the 3'non-coding region of CCND1 contained the binding site of miR-138.Luciferase assay showed that miR-138 could regulate CCND1.Conclusion The miR-138 could inhibit the proliferation,migration and invasion of renal cancer cells,and its mechanism might be related to the targeted regulation of CCND1.
miRNA-138renal cancer cellscell proliferationcell migrationcell invasioncyclin D1
国家科技期刊平台
NSTL
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