Effects of subcutaneous injection of GLP-1 receptor agonist liraglutide on serum sex hormones in obese male mice
Objective To investigate the effects of subcutaneous injection of glucagon-like peptide-1(GLP-1)re-ceptor agonist liraglutide on serum sex hormone levels in obese male mice.Methods Eighteen male C57BL/6J mice were randomly divided into the normal group(n=6),model group(n=6),and intervention group(n=6).Mice in the nor-mal group were fed with normal diet,and mice in the model group and intervention group were fed with high-fat diet to es-tablish the obesity models.After 10 weeks,mice in the intervention group were subcutaneously injected with liraglutide at 100 μg/(kg·d),and mice in the normal group and model group were subcutaneously injected with the equal volume of nor-mal saline for 12 weeks.Body weight was measured weekly.Fasting blood glucose(FBG)was measured using a blood glu-cose test strip.The serum levels of insulin(INS),luteinizing hormone(LH),follicle-stimulating hormone(FSH),testos-terone(T),free testosterone(FT),estradiol(E2),and sex hormone binding globulin(SHBG)were detected using ELI-SA.The homeostasis model assessment of insulin resistance(HOMA-IR)was calculated.Results Compared with the normal group,the body weight of the model group increased at 0,4,8,and 12 weeks(all P<0.01);compared with the model group,the body weight of the intervention group decreased at 4,8,and 12 weeks(all P<0.01);there was no statis-tically significant difference in body weight between the intervention group and the normal group at 12 weeks(P>0.05).After administration,compared with the normal group,the serum FBG,INS,and HOMA-IR levels increased in the model group(all P<0.01);compared with the model group,the serum FBG,INS,and HOMA-IR levels decreased in the inter-vention group(all P<0.01).Compared with the normal group,the levels of LH,FSH,T,and FT decreased and the lev-els of E2 and SHBG increased in the model group(all P<0.05);compared with the model group,the levels of LH,FSH,T,and FT in the intervention group increased,and the levels of E2 and SHBG decreased(all P<0.05).Conclusion The sex hormone levels of obese male mice are abnormal;liraglutide can improve the sex hormone levels of obese male mice,and its mechanism may be related to its significant effects of reducing blood glucose and body mass.
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