Comparison on osteogenic effect of nano-hydroxyapatite/collagen and calcined natural bovine bone repair materials in the defect repair of skull in rats
Objective To compare and observe the osteogenic effects of nano-hydroxyapatite/collagen and natural calcined bone repair materials in the defect repair of skull in rats.Methods Eighteen SD rats were randomly divided in-to three groups,with 6 rats in each group.The bilateral critical size bone defect models of SD rats were established in both groups,and the defect repair operation was performed.During the operation,the defect site on one side of the skull was filled with nano-hydroxyapatite/collagen repair material(nano-hydroxyapatite/collagen repair material group)and on the other side was filled with calcined natural bovine bone(calcined natural bovine bone group).At the 4th,8th and 12th weeks after surgery,the new bone tissues of the bilateral skull defect of rats were taken,and the osteogenesis effects were evaluated by Micro-CT and pathological examination results[osteogenesis velocity:the bone volume/tissue volume(BV/TV)and the bone volume fraction(BVF);osteogenesis quality:trabecular number(Tb.N),trabecular thickness(Tb.Th),and trabecular space(Tb.Sp),the number of fibrous connective tissues,the number of new capillaries in new bone tissues],and they were compared.Results Micro-CT results showed:at 4,8 and 12 weeks after operation,the BV/TV,Tb.Th and Tb.N of the new bone in the nano-hydroxyapatite/collagen repair material group were higher,and the Tb.Sp was lower than those in the calcined natural bovine bone group.HE staining results showed:at 4,8 and 12 weeks after operation,the BVF of the new bone in the nano-hydroxyapatite/collagen repair material group was higher than that of the calcined natural bovine bone group,but the nano-hydroxyapatite/collagen was absorbed quickly,the new bone contained more connective tissue and blood vessels,and the bone was softer.The absorption rate of calcined natural bovine bone was slow,the connective tissue and blood vessels in the new bone were less,and the bone was harder.Conclusions In the repair of skull critical size defect in rats,the osteogenesis velocity of nano-hydroxyapatite/collagen repair material is fast-er,the osteogenesis quality is higher,the absorption is faster,the new bone tissues have more connective tissue and new blood vessels,and the new bone is similar to"cancellous bone".The osteogenesis velocity of calcined natural bovine bone is slower,the connective tissues and new blood vessels in the new bone tissues are less,and the new bone is similar to"cor-tical bone".
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