Effects and mechanism of Three-Manipulation and Three-Acupoint Tuina on immediate analgesia in rats with sciatic nerve injury
Objective To observe the immediate analgesic effects of Three-Manipulation and Three-Acupoint Tuina on rats with sciatic nerve injury and to explore its mechanisms.Methods Sprague-Dawley(SD)rats were randomly di-vided into the sham surgery group,model group,and Tuina group,with six in each group.Rats in the model and Tuina groups were subjected to the mild chronic constriction injury of the sciatic nerve to simulate clinical neuropathic pain,while rats in the sham surgery group only had nerve exposure followed by suturing.Seven days after modeling,rats in the Tuina group received Three-Manipulation and Three-Acupoint Tuina intervention using a massage simulator,whereas rats in the sham surgery and model groups underwent equal duration of grip restraint.Mechanical withdrawal threshold(MWT)and thermal withdrawal latency(TWL)were measured before modeling(T1),before Tuina(T2),and after Tuina(T3)to evaluate the immediate analgesic effects of Three-Manipulation and Three-Acupoint Tuina.Subsequently,spinal dorsal horn tissues in intumescentia lumbalis were collected for detection of P2Y12 purinergic receptor(P2RY12),phosphorylat-ed p38 mitogen-activated protein kinase(p-p38 MAPK),and tumor necrosis factor-α(TNF-α)proteins via Western blot-ting.Results Compared with the sham surgery group at the same time points,MWT and TWL in the model and Tuina groups decreased at T2 and T3(all P<0.05);compared with the model group at the same time points,MWT and TWL in the Tuina group increased at T3(P<0.05).The expression levels of P2RY12,p-p38 MAPK,and TNF-α proteins in the model group were higher than those in the sham surgery and Tuina groups(all P<0.05),with no significant difference be-tween the sham surgery and Tuina groups(all P>0.05).Conclusion Three-Manipulation and Three-Acupoint Tuina ex-hibits significant immediate analgesic effects in rats with sciatic nerve injury,potentially through inhibiting the microglial P2RY12/p38 MAPK/TNF-α pathway,thereby alleviating neuropathic pain.
万方数据
维普
