Effect of methyltransferase SETDB1 on migration and invasion of oral cancer cells and its mechanism
Objective To observe the effect of methyltransferase SETDB1 on the migration and invasion of oral squa-mous cell carcinoma cells,and to explore the related mechanisms.Methods Different concentrations of methyltransfer-ase inhibitors GSK3685032(0,0.2,0.4,0.6,0.8 µmol/L)acted on the oral squamous cell carcinoma CAL-27 cells.Cell proliferative viability was measured by the MTT method,and we found that the optimal action concentration of GSK3685032 was 0.6 µmol/L.CAL-27 cells were divided into the control and experimental groups;cells in the control group were treated with organic solvent DMSO,and cells in the experimental group were treated with 0.6 µmol/L GSK3685032.Cell SETDB1 mRNA was measured by RT-qPCR,and the SETDB1 protein was detected by Western blot-ting.Cell migration ability was detected by Scratch assay,the invasion ability was detected by Transwell chamber invasion assay,and SOX 7 methylation level was tested by pyrosequencing.Results The mRNA and protein expression levels of SETDB1 were lower in the experimental group than in the control group(both P<0.05).The migration distance of the ex-perimental group was less than that of the control group,and the number of transmenbrance cells was smaller than that of the control group(P<0.05).The methylation rate of SOX 7 in the experimental group was lower than that of the control group(P<0.05).Conclusion Methyltransferase SETDB1 can inhibit the migration and invasion of oral squamous cell carcinoma cells,and the mechanism may be related to the down-regulation of intracellular SOX7 methylation level.
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