Proliferation,migration and invasion abilities of pancreatic cancer cells with over-/down-expression of circSTON2
Objective To observe change of proliferation,migration and invasion abilities of pancreatic cancer cells with over-/down-expression of circSTON2,and to preliminarily investigate the biological function of circSTON2 in pancre-atic cancer.Methods Human pancreatic cancer cell line PANC-1 was in vitro cultured.CircSTON2 over-expression group,circSTON2 down-expression group,and control group were established.Plasmid transfection and siRNA interfer-ence were used to transfect the circSTON2 overexpression plasmid and circSTON2-siRNA interference plasmid and blank control plasmid into the above groups.At 24,36 and 48 hours of culture,CCK-8 assay was used to detect cell proliferation and Transwell assay was used to detect cell migration and invasion.Results At 24,36 and 48 hours of culture,cell pro-liferation ability was as follows:circSTON2 over-expression group>control group>circSTON2 down-expression group(all P<0.05);the number of migration cells and the number of invasion cells were as follows:circSTON2 over-expression group>control group>circSTON2 down-expression group(all P<0.05).Conclusions The over-expression of circS-TON2 promotes proliferation,migration,and invasion of pancreatic cancer cells.However,the effect of down-expression of circSTON2 is the opposite.
万方数据
维普
