Objective To observe the ameliorative effect of ethanol extract of semen cuscutae on postmenopausal os-teoporosis in rats and to explore its mechanism.Methods Female SD rats were randomly divided into the normal group,sham operation group,model group,estrogen control group,low-dose semen cuscutae group,and high-dose semen cuscu-tae group,with 10 rats in each group.In addition to normal group and sham operation group,bilateral ovariectomy was per-formed in all groups to establish postmenopausal osteoporosis models.In the sham operation group,the adipose tissues around the ovary were removed after the ovary was exposed,but the ovary was not removed.Rats in the normal group were fed without any treatment.Starting from two weeks after modeling,rats in the low-dose semen cuscutae group and high-dose semen cuscutae group were given ethanol extract of semen cuscutae by gavage at 0.7 and 1.4 g/kg,respectively;rats in the estrogen control group were given non-steroidal estrostilbestrol by gavage;rats in the normal group,sham operation group and model group were given equal volume of normal saline by gavage for 12 weeks.The bone mineral density(BMD)of rat femur was detected by dual-energy X-ray,and the pathological morphology of femur was observed by HE staining.The miR-21-5p of femur was detected by RT-PCR,and Fas ligand(FasL)protein of femur was detected by West-ern blotting.Results The bone mineral density of femur in the model group was lower than that in the normal group and sham operation group,and the bone mineral density of femur was higher in the estrogen control group,low-dose semen cus-cutae group and high-dose semen cuscutae group than in the model group(all P<0.05).Cortical periosteum and endoste-um were smooth,trabecular shape was basically the same,and only slight cancellous bone changes appeared in the corti-cal area.In the model group,bone trabeculae became thinner,interval became wider,disconnected and was disordered,and there were serious cancellous bone changes in the cortical region.Compared with the model group,the bone trabecular distance decreased to different degrees,and the cortical cancellous bone changes became lighter in the estrogen control group,low-dose semen cuscutae group and high-dose semen cuscutae group.The expression of miR-21-5p in femur tissues of the model group was higher than that of the normal group and sham operation group,and the expression of miR-21-5p in the estrogen control group,low-dose semen cuscutae group and high-dose semen cuscutae group was lower than that of mod-el group(all P<0.05).The expression of FasL protein in femur tissues of the model group was lower than that of the nor-mal group and sham operation group,and that of estrogen control group,low-dose semen cuscutae group and high-dose se-men cuscutae group was higher than that of the model group(all P<0.05).Conclusion Ethanol extract of semen cuscu-tae can improve postmenopausal osteoporosis in rats,and the mechanism may be related to the regulation of miR-21-5p and its downstream protein FasL.
关键词
菟丝子/菟丝子乙醇提取物/绝经后骨质疏松症/微小RNA-21-5p/Fas配体
Key words
Semen Cuscutae/alcohol extract of Semen Cuscutae/postmenopausal osteoporosis/microRNA-21-5p/Fas ligand
维普
万方数据