Anti-inflammatory and antinociceptive effects and acute toxicity of the ethyl acetate fraction of Fissistigma polyanthum
Objective To investigate the anti-inflammatory and antinociceptive effects and acute toxicity of the ethyl acetate fraction(FPEA)from the 95%ethanol extraction of Fissistigma polyanthum(FPEE)in vivo.Methods Observa-tion of anti-inflammatory effects:ICR mice were randomly divided into the blank control group,model group,positive group,and low-,medium-,and high-dose FPEA groups.Mice in low-,medium-,and high-dose FPEA groups were ga-vaged with 200,400,and 800 mg/kg of FPEA,respectively.Mice in the positive group were gavaged with dexametha-sone,while mice in the blank control group and model group were gavaged with equal volume of normal saline.Mice were treated daily for five consecutive days.Except for the blank control group,the right hind paw of each mouse was subcutane-ously injected with carrageenan to induce edema.The paw volume was measured at 1,2,3,4,5,and 6 h after the injec-tion,then the edema degree and the inhibition rate of paw edema were calculated.The levels interleukin-1β(IL-1β),IL-6,and tumor necrosis factor-α(TNF-α)in serum of the mice at 6 h after the injection were measured by ELISA.ICR mice were randomly divided into model group,positive group,and low-,medium-,and high-dose FPEA groups,and mice in each group were treated with the same methods as above.The ear edema degree and the inhibition rate of ear edema were calculated using the xylene-induced ear edema model.Observation of antinociceptive effects:ICR mice were randomly di-vided into the model group,positive group,and low-,medium-,and high-dose FPEA groups.Mice in each group were treated with the same methods as described above,but mice in the positive group were treated with the aspirin and Yuanhu Zhitong tablets.Each mouse was given an intraperitoneal injection of acetic acid,then the number of abdominal writhing was recorded for 30 min and the inhibition rate of writhing was calculated.The latency time was determined at 30,60,90,and 120 min after the drug administration.Observation of acute toxicity:ICR mice were randomly divided into the blank control group,FPEE group,and FPEA group(half male and half female).Mice in the FPEE group and FPEA group were gavaged with FPEE and FPEA at a dose of 2000 mg/kg,respective,while mice in the blank control group were gavaged with equal volume of normal saline.The body weight of mice were recorded within 14 days after intragastric administra-tion.At the end of the observation,all mice were sacrificed,then the organ indexes of heart,liver,spleen,lung,kidney,and thymus were calculated.Results Compared with the model group,the paw edema degree of mice in the positive group were reduced at 4,5,and 6 h after the induction of inflammation,and the paw edema degree of mice in medium-and high-dose FPEA groups were reduced at 5 and 6 h after the induction of inflammation(all P<0.05).At 6 h after the in-duction of inflammation,there was no significant difference in the inhibition rate of paw edema between the high-dose FPEA and positive groups(P>0.05).The levels of IL-1β,IL-6,and TNF-α in serum of the model group were higher than those of the blank control group,the levels of IL-6 and TNF-α in the positive group and low-,medium-,and high-dose FPEA groups were lower than those of the model group,and the level of IL-1β in the positive group and medium-and high-dose FPEA groups were lower than those of the model group(all P<0.05).The ear edema degree were lower in the posi-tive group and low-,medium-,and high-dose FPEA groups than in the model group(all P<0.05),and there was no signif-icant difference in the in the inhibition rate of ear edema between the medium-and high-dose FPEA groups and the positive group(all P>0.05).The writhing times of mice in the positive group and low-,medium-,and high-dose FPEA groups were less than those in the model group,and the inhibition rates of writhing in low-,medium-,and high-dose FPEA groups were less than that of the positive group(all P<0.05).The latency time at 60,90,and 120 min in the positive group and low-,medium-,and high-dose FPEA groups were longer than that of the model group(P<0.05).There was no significant difference in the body weight or organ indexes of heart,liver,spleen,lung,kidney,and thymus between the treated(at the dose of 2000 mg/kg)and the blank control groups(all P>0.05).Conclusion The FPEA exhibited better anti-in-flammatory and antinociceptive effects,and did not show acute toxicity.
万方数据
维普
