Induction results of CCEC on systemic allergic reactions in KM mice,and anaphylactoid reaction in rat peritoneal mast cells and RBL-2H3 cells
Objective To observe the induction results of Child Compound Endothelium Comeum(CCEC)on sys-temic allergic reactions in KM mice and anaphylactoid reaction in rat peritoneal mast cells(RPMC)and basophilic leuke-mia cell line RBL-2H3 in Wistar rats.Methods(1)Forty-eight KM mice were randomly divided into groups A,B,C and D.Mice in the group A were given 0.25g crude drug/kg CCEC suspension,once every other day for three consecutive times,and they were gavaged again at intervals of 9 and 16 days,respectively;mice in the group B were given 0.025 g crude drug/kg CCEC suspension,once every other day for three consecutive times,and they were gavaged again at inter-vals of 9 and 16 days,respectively;mice in the group C were given 1 mg ovalbumin(OVA)intravenously,once every oth-er day for three consecutive times,and they were gavaged again at intervals of 9 and 16 days,respectively;mice in the group D were given intravenous injection of 0.1 mL/10 g normal saline,once every other day for three consecutive times,and they were gavaged again at intervals of 9 and 16 days,respectively;the active systemic allergic reaction of rats in each group was observed.(2)Primary RPMC were isolated from the abdominal cavity of another 10 SD rats and were divided in-to five groups:the blank control group,C48/80 group,high-,medium-and low-dose groups;cells in the high-,medi-um-,and low-dose groups were added with 32,16 and 8 mg/mL of CCEC suspension,respectively.The cells of the C48/80 group and blank control group were added with 0.04 mg/mL of histamine release agent and mast cell degranulation agent C48/80 and buffer solution,respectively;the release rate and promotion rate of histamine and β-amino-hexosidase were measured in each group.(3)RBL-2H3 cells in the logarithmic growth phase were divided into the blank control group,C48/80 group and high-,medium-and low-dose groups.The cells in high-,medium-and low-dose groups were added with 32,16 and 8mg/mL of CCEC suspension,respectively,and the cells in C48/80 group and blank control group were added with 0.04 mg/mL of C48/80 and buffer solution,respectively.The release rate and promotion rate of hista-mine and β-amino-hexosidase were measured in each group.Results On the 14th and 21st days,no anaphylaxis oc-curred in the groups A,B,and D,and the anaphylaxis was negative.The mice in the group C had dyspnea,death and strong positive allergic reaction.The release rate of histamine in the C48/80 group was 72.6%and the release rates and promotion rates of histamine in the high-,medium-and low-dose groups were lower than 64.23%.The release rate and promotion rate of β-aminoglycosidase were 110.53%in the C48/80 group(positive sensitization),and the release rates and promotion rates of β-aminoglycosidase were lower than 80.31%in the high-,medium-and low-dose groups(no sensi-tizing effect).The release rate and promotion rate of histamine in the RBL-2H3 cells of the C48/80 group were 81.6%,and the release rates and promotion rates of the high-,medium-and low-dose groups were lower than 64.23%.The re-lease rate and promotion rate was 120.93%in the C48/80 group(positive sensitization),and the release rates and promo-tion rates of β-aminoglycosidase were lower than 80.31%in the high-,medium-and low-dose groups(no sensitizing ef-fect).Conclusions CCEC neither induced systemic allergic reactions in KM mice nor caused anaphylactoid reactions in primary RPMC and RBL-2H3 cells of basophilic leukemia cell line from Wistar rats.
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