Expression changes of CDCA5 gene in cervical cancer tissues and cells and its effect on proliferation of cervical cancer cells
Objective To observe the expression changes of the CDCA5 gene in the cervical cancer tissues and cells,to observe the effect of its overexpression on the proliferation of cervical cancer cells,and to investigate the potential underlying molecular mechanism.Methods Sixty pairs of cervical cancer tissues and adjacent tissues were obtained for analysis,along with the immortalized human cervical epithelial cell line H8 and human cervical cancer cell lines SiHa,C-33A,MS751,and HeLa,which were cultured for experimentation.The levels of CDCA5 mRNA in both the tissues and cells were assessed using real-time fluorescent quantitative PCR.The cervical cancer cells exhibiting the lowest relative expression of CDCA5 mRNA were randomly assigned into the control and overexpression groups,which were transfected with pCDH empty vector and CDCA5 overexpressed vector,respectively.Cell proliferation was assessed using CCK-8 and clone formation tests,while Western blotting was employed to detect the p-ERK and p-AKT protein.Results The relative expression level of CDCA5 mRNA in the cervical cancer tissues was higher than that in the adjacent tissues(P<0.05);the expression of CDCA5 mRNA in cervical cancer cell lines SiHa,C-33A,MS751,and HeLa was higher than that in H8 cells,among which the relative ex-pression level was the lowest in HeLa cells(all P<0.05).The cell proliferation abilities at 24,48,and 72 h were higher in the overexpression group than in the control group,and the number of cell plate clone formation and the expression of p-ERK and p-AKT proteins in the overexpression group were higher than those in the control group(all P<0.05).Conclusion CDC-DA5 gene is highly expressed in cervical cancer tissues and cells,and the overexpression of CDCDA5 gene can enhance the proliferation of human cervical cancer cells,which may be related to the regulation of ERK/AKT signaling pathway.
cell division cycle-associated protein 5cervical carcinomacell proliferationERK/AKT signaling pathway
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