Mechanism of progesterone in inhibiting neuron damage of neonatal mice subjected to OGD/R based on the regulation of pgrmc1
Objective Based on the regulation of progesterone membrane receptor component 1(pgrmc1),to ex-plore the inhibition mechanism of progesterone(PROG)on neuronal damage of newborn mice subjected to oxygen glucose deprivation/reoxygenation(OGD/R).Methods Primary cortical neuronal cells were isolated from newborn mice within 12 hours of birth,cultured in vitro for 7 days,and were identified using microtubule-associated protein 2(MAP2)detec-tion.The neuronal cells were randomly divided into the control group,dimethyl sulfoxide(DMSO)group,AG205 group,PROG group,and AG205+PROG group,respectively.Neuronal cells in the DMSO group,AG205 group,PROG group,and AG205+PROG group were added with prepared glucose deficient D-Hanks solution and were cultured in an hypoxic incubator for 2 h,then were replaced with neuronal growth medium.At 1 h before OGD/R models were established,cells in the DMSO group were treated with DMSO,and cells in the AG205 and AG205+PROG groups were treated with AG205(10 μmol/L).At 2 h after OGD/R models were established,cells in the AG205+PROG group were treated with PROG(20 μmol/L).After 24 h of reoxygenation,cell viability was detected using CCK-8,and apoptotic cells were detected us-ing flow cytometry.Results The cell survival rates of the DMSO group and the AG205 group were lower than that of the control group,and that was lower in the AG205 group than in the DMSO group.The cell survival rates of the PROG group and the AG205+PROG group were higher than that of the AG205 group,and that was higher in the PROG group than in the AG205+PROG group(all P<0.05).The apoptosis rates of the DMSO group and the AG205 group were higher than that of the control group,and that was higher in the AG205 group than in the DMSO group;the apoptosis rates of the PROG group and the AG205+PROG group were lower than that of the AG205 group(all P<0.05).Conclusion PROG can inhibit neuronal damage in OGD/R neonatal mice,inhibiting pgrmc1 can reduce OGD/R neuronal activity and in-crease apoptosis,and its mechanism may be related to the regulation of pgrmc1.
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