Predictive efficacy of relative expression of serum LncRNA-ZFAS1 and miR-15a-5p for poor prognosis in patients with status epilepticus
Objective To observe the changes in the expression levels of serum LncRNA-ZFAS1 and miR-15a-5p in patients with status epilepticus(SE),and to analyze the predictive efficacy of the relative expression of serum LncRNA-ZFAS1 and miR-15a-5p for the poor prognosis of SE patients.Methods Totally 100 cases of SE patients(SE group)and 68 cases of healthy people with physical examination in the same period(control group)were selected,and real-time fluo-rescence quantitative polymerase chain reaction was used to detect serum LncRNA-ZFAS1 and miR-15a-5p levels in the subjects,and the binding sites of LncRNA-ZFAS1 and miR-15a-5p were predicted by starBase database.General data such as gender,age,disease duration,seizure type,underlying disease,in-hospital complications,brain tissue abnormal-ities,cerebrospinal fluid abnormalities,electroencephalogram abnormalities,concomitant fever,history of≥2 previous seizures,duration of epilepsy,mechanical ventilation,status epilepticus severity score(STESS),time in the intensive care unit,length of hospital stay,and types of therapeutic medications were collected from the patients with SE.According to the Glasgow Prognostic Score,the SE patients were divided into the poor prognosis group of 31 cases and the good prog-nosis group of 69 cases.We compared the general data and the relative expression levels of serum LncRNA-ZFAS1 and miR-15a-5p of the patients in the poor prognosis group and the good prognosis group.Risk factors for poor prognosis in SE patients were analyzed by multifactorial Logistic regression analysis.Using poor prognosis in SE patients as the dependent variable,and variables that were statistically different in univariate analyses as independent variables.The risk factors for poor prognosis in SE patients were analyzed using multivariate Logistic regression analysis.The predictive efficacy of the combination of relative expression of serum LncRNA-ZFAS1 and miR-15a-5p on poor prognosis in SE patients was ana-lyzed using receiver operating characteristic(ROC)curve.Results The relative expression of serum LncRNA-ZFAS1 in the SE group was higher than that of the control group,and the relative expression of miR-15a-5p was lower than that of the control group(both P<0.05).There was a negative correlation between the relative expression of serum LncRNA-ZFAS1 and miR-15a-5p in the SE patients(r=-0.742,P<0.05).LncRNA-ZFAS1 had a complementary sequence to the 3'un-translated end of miR-15a-5p.The convulsive SE,seizure duration≥1 h,the proportion of mechanical ventilation,STESS score,and serum LncRNA-ZFAS1 were higher in the poor prognosis group than in the good prognosis group,and the rela-tive expression of serum miR-15a-5p was lower than that of the good prognosis group(all P<0.05).The AUC of serum Ln-cRNA-ZFAS1 in predicting poor prognosis of SE patients was 0.786,with a sensitivity of 67.74%and a specificity of 75.36%;the AUC of serum miR-15a-5p in predicting poor prognosis of SE patients was 0.781,with a sensitivity of 90.32%and a specificity of 53.62%;and the AUC of serum LncRNA-ZFAS1 combined with miR-15a-5p in predicting poor prognosis of SE patients was 0.879,with the sensitivity of 87.10%and specificity of 73.91%.Conclusions Se-rum LncRNA-ZFAS1 is highly expressed and miR-15a-5p is low expressed in SE patients,which shows a negative correla-tion.Elevated relative expression of serum LncRNA-ZFAS1 is an independent risk factor for poor prognosis of SE patients,and elevated relative expression of serum miR-15a-5p is an independent protective factor.Detection of serum LncRNA-ZFAS1 and miR-15a-5p can be used for the prediction of poor prognosis in SE patients,and the predictive value of the two combined is higher.
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